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通过正规/非正规 Wnts 和 Tlr4/NFκBB 之间的负反循环来确定斑马鱼背部组织器的大小

Juqi Zou¹, Satoshi Anai², Satoshi Ota³

¹Department of Homeostatic Regulation, Division of Cellular and Molecular Biology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, 565-0871, Japan.

Nature communications

|November 8, 2023

概括

在斑马鱼中,一个涉及Wnt配体和Toll类受体4 (Tlr4) / NFκB信号的反循环调节了组织者大小. 这一途径通过限制Wnt-依赖的组织者形成来控制背腔腹腔模式.

科学领域:

发育生物学是发展生物学.
分子信号通道的分子信号通道.
比較胚胎學比較胚胎學

背景情况:

脊椎动物的背部组织者,对于胚胎模式至关重要,由Wnt配体进行初始化,并分泌BMP抗剂.
果虫胚胎发生利用托尔类受体 (Tlr) 介导的NFκB激活用于背腔腹腔模式,Wnt反调节了这一过程.
在脊椎动物组织者形成中Tlr/NFκB和反循环的作用仍然在很大程度上未被阐明.

研究的目的:

研究TLr/NFκB和Wnt反在斑马鱼组织者形成中的参与.
阐明控制斑马鱼组织者大小的分子机制.
为了比较Wnt和Tlr/NFκB信号传递在Drosophila和斑马鱼发育中的作用.

主要方法:

斑马鱼胚胎的现场成像.
斑马鱼的遗传修饰技术.
对Wnt和Tlr4/NFκB信号通路的分析.

主要成果:

确定了正规和非正规Wnts和Tlr4 / NFκB之间的负反循环,确定了斑马鱼组织者大小.
Wnt和Tlr/NFκB信号显示了Drosophila和斑马鱼之间的初始线索和反媒介的交换角色.
规范性Wnt信号调节非规范性Wnt5b,激活Tlr4/NFκB转录Wnt对手frzb,从而限制组织者形成.

结论:

这项研究揭示了一种新的反机制,通过Wnt和Tlr4/NFκB信号调节斑马鱼组织者大小.
对比分析强调了这些途径在无脊椎动物和脊椎动物发育中的保留和分离作用.
这种复杂的信号网络确保精确控制胚胎模式和组织者发展.

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