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克西兰的使用促进了Bifidobacterium pseudocatenulatum适应人类胃肠道的发生

Yohei Watanabe¹, Yuki Saito², Taeko Hara²

¹Yakult Central Institute, Kunitachi, Tokyo, Japan. yohei-watanabe@yakult.co.jp.

ISME communications

|November 8, 2023

概括

饮食中的长链西兰 (LCX) 在肠道中促进Bifidobacterium pseudocatenulatum的生长. BpXyn10A基因的存在对这种生长和细菌竞争力至关重要.

科学领域:

微生物学微生物学
人类肠道微生物群
饮食纤维的新陈代谢

背景情况:

饮食中的碳水化合物显著影响肠道微生物群的组成.
碳水化合物可用性对人类肠道单个细菌生长的具体影响尚未完全理解.
长链西兰 (LCX) 是丰富的食纤维,对特定的肠道细菌有很大程度上不具特征的作用.

研究的目的:

研究长链西兰 (LCX) 在促进Bifidobacterium pseudocatenulatum在成年人肠道中的生长中的作用.
确定B. pseudocatenulatum.使用LCX的基因和分子机制.
评估LCX在肠道环境中的利用所带来的竞争优势.

主要方法:

对B. pseudocatenulatum菌株的基因组和表型分析.
对内分泌-1,4-β-xylanase (BpXyn10A) 基因及其在LCX分解中的作用的研究.
共同培养实验与西兰分解性Bacteroides物种进行.
在人类的饮食LCX干预期间分析B. pseudocatenulatum水平.

主要成果:

在成年人肠道中,LCX的可用性显著促进了B. pseudocatenulatum的生长.
内分-1,4-β-xylanase (BpXyn10A) 基因通过裂解纤维素骨干使LCX利用成为可能,尽管并非所有菌株都具有这种基因.
使用LCX的菌株表现出更强的竞争力,即使有预先消化的LCX寡糖.
人类内源性B. pseudocatenulatum水平的增加与LCX丰富饮食期间BpXyn10A基因的存在相关.

结论:

在人类肠道中,LCX的可用性是B. pseudocatenulatum的关键适应性决定因素.
聚糖的利用,特别是通过BpXyn10A的LCX分解,增强了双菌的肠道适应性.
参与多糖分解的关键酶可以作为生物标志物,通过饮食干预来操纵肠道微生物群.

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