Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

pCODE tRNA Expression Plasmids Compensate for Rare Codons in Recombinant Protein Production.

ACS synthetic biology·2026
Same author

NrdR in Streptococcus and Listeria spp.: DNA Helix Phase Dependence of the Bacterial Ribonucleotide Reductase Repressor.

Molecular microbiology·2025
Same author

Production of recombinant coiled coil silk proteins for materials synthesis.

Protein expression and purification·2025
Same author

Biosensor that Detects Stress Caused by Periplasmic Proteins.

ACS synthetic biology·2024
Same author

Structural and functional insights into the Pseudomonas aeruginosa glycosyltransferase WaaG and the implications for lipopolysaccharide biosynthesis.

The Journal of biological chemistry·2023
Same author

Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli.

Current genetics·2023
Same journal

Is heptelidic (koningic) acid a microbial hormone that regulates secondary metabolism in the biocontrol fungus Trichoderma virens?

Current genetics·2025
Same journal

Loss of PDR3 alters metabolome in response to MCHM, a synthetic hydrotrope.

Current genetics·2025
Same journal

The CRISPR-cas repertoire of Kluyvera ascorbata: insights from genomic data.

Current genetics·2025
Same journal

Genome characterization of Acinetobacter species from the rice rhizosphere: a potential plant growth promoting rhizobacteria (PGPR).

Current genetics·2025
Same journal

Genomic surveillance of vancomycin-resistant Enterococcus faecium: a study on Resistome, Plasmidome, and mobilome profiling.

Current genetics·2025
Same journal

Epigenetic regulation of pathogenicity in fungi.

Current genetics·2025
查看所有相关文章

相关实验视频

Updated: Jul 11, 2025

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
07:08

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Published on: December 23, 2015

13.8K

如何有效地维护pET表达等离子体的技巧

Diana Khananisho1, Alister J Cumming1, Daria Kulakova1

  • 1Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.

Current genetics
|November 8, 2023
PubMed
概括
此摘要是机器生成的。

通过选择正确的Escherichia coli宿主菌株和抗生素耐药性磁带,有效地维持用于重组蛋白质生产的PET等离子体. 优化诱导时间以防止等离子体损失,特别是高T7RNA聚合酶菌株.

关键词:
氨基糖化物-3-光转移酶的氨基糖化物-3-光转移酶.一个细菌细胞工厂.等离子体的不稳定性质粒维护维护 质粒维护质粒的稳定性 质粒的稳定性Tn3 Tn3 的时间时间9033天.的pET表达等离子体.这是一种β-Lactamase.

更多相关视频

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.4K
Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
11:24

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Published on: March 25, 2015

10.0K

相关实验视频

Last Updated: Jul 11, 2025

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
07:08

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Published on: December 23, 2015

13.8K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.4K
Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
11:24

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Published on: March 25, 2015

10.0K

科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 微生物学 微生物学

背景情况:

  • pET表达等离子体对于大肠杆菌的重组蛋白质生产至关重要.
  • 血维护依赖于抗生素耐药性标记物,通常是β-乳糖酶 (Tn3.1) 或氨基糖类转化酶 (Tn903.1).

研究的目的:

  • 用Tn3.1和Tn903.1遗传片段研究pET等离子体维护的效率.
  • 为了确定影响长期重组蛋白表达过程中等离子体稳定性的因素.

主要方法:

  • 在不同的大肠杆菌菌株 (例如,C41(DE3),BL21(DE3) 中对pET等离子体维持的比较分析.
  • 评估不同诱导时间 (短时间与长时间) 和抗生素选择压力下的等离子体稳定性.
  • 对Tn3.1和Tn903.1磁带性能进行评估.

主要成果:

  • 在短的诱导时间下,pET等离子体在两个电阻标记器下有效地维持.
  • 较长的诱导时间 (20小时) 显示了压力依赖的等离子体维护.
  • 等离子体的稳定性受宿主菌株和使用的特定抗生素耐药性磁带的影响.

结论:

  • 菌株选择 (例如,低T7RNA聚合酶的C41~DE3) 确保在长时间的诱导期内有效地维持等离子体.
  • 对于高T7RNA聚合酶菌株 (例如,BL21(DE3),建议使用短诱导或Tn903.1录音带与kanamycin选择,以保持稳定的pET等离子体.