多种药物排泄 通过系统介导的黄金葡萄球菌在一个健康方法下的耐药性
Mariana de Barros1, Ilderlane da Silva Lopes1, Ana Júlia Moreira1
1Department of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
World journal of microbiology & biotechnology
|November 8, 2023
概括
携带活性多药物排泄系统 (MES) 的多药物耐药黄金葡萄球菌 (Staphylococcus aureus) 正在在人类,动物和食品领域蔓延. 这凸显了在"一个健康"框架内,需要制定制耐药性传播的战略.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 抗菌素耐药性 (AMR) 是一个日益增长的全球健康威胁.
- 多种药物排放系统 (MES) 在细菌产生抗菌素耐药性 (AMR) 方面发挥着至关重要的作用.
- 了解AMR的一种健康传播对于有效的控制策略至关重要.
研究的目的:
- 调查多药物排泄系统 (MES) 在多药物耐药 (MDR) 和甲素耐药金黄色葡萄球菌 (MRSA) 隔离物中的流行率和活性.
- 评估人类,动物和食品部门之间MDR/MRSA传播的可能性.
- 评估排泄活动对生物膜形成的影响.
主要方法:
- 使用PCR对排泄基因 (norA,norB,norC,LmrS,tet38,msrA) 的查.
- 测定带有和没有CCCP的四环素和西普洛素的最小抑制度 (MIC).
- 量化乙基化物外流和评估生物膜的形成.
- 使用脉冲场凝电泳 (PFGE) 进行分子类型化.
主要成果:
- 在所有分离物体中检测到norC基因,而msrA只在两个人类分离物体中发现.
- 在55.9%的隔离物体中观察到乙化排放,这表明有活跃的排放.
- CCCP的存在减少了生物膜的形成,PFGE揭示了来自不同来源的分离物的混合集群.
结论:
- 具有活跃的多种药物排放系统的抗多种药物黄金葡萄球菌正在人类,动物和食品环境中传播.
- 一个健康部门的相互联系促进了抗菌素耐药性的传播.
- 干预策略是必要的,以限制MES介导的耐药性的循环和传播.
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