相关实验视频
Updated: Jul 11, 2025
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Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
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Circ_ZNF778_006通过通过海绵化miR-18b-5p对HIF-1α表达进行上调来促进ESCC进展
Xianzhe Si1, Xincheng Su2, Weijie Lin1
1Department of Gastrointestinal and Esophageal Surgery, The 2nd Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian Province, China.
Scientific reports
|November 8, 2023
概括
循环RNA ZNF778 (circ_ZNF778_006) 通过通过海绵化miR-18b-5p增加HIF-1α,抑制化疗敏感性,促进食道状细胞癌 (ESCC) 的进展.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 循环RNAs (circRNAs) 与各种癌症有关,之前发现 circ_ZNF778_006 在食道状细胞癌 (ESCC) 中升高.
- circ_ZNF778_006在ESCC病变发生中的确切作用及其分子机制在很大程度上仍未被阐明.
研究的目的:
- 研究circ_ZNF778_006在ESCC进展中的功能作用和潜在的分子机制.
- 探索ESCC中circ_ZNF778_006,miR-18b-5p和HIF-1α之间的关系.
主要方法:
- 使用定量实时PCR (qRT-PCR) 和西班牙血栓来分析表达水平.
- 用CCK8和Transwell测定来评估细胞增殖,迁移和入侵.
- 在体内进行了瘤生长,光酶记者测定和RNA结合蛋白免疫沉 (RIP).
主要成果:
- Circ_ZNF778_006表达与ESCC病理等级和对5-甲和西斯普拉丁敏感性降低正相关.
- Circ_ZNF778_006促进了ESCC细胞的增殖,入侵,迁移,并在体内加速了瘤的生长.
- Circ_ZNF778_006通过海绵化miR-18b-5p对HIF-1α进行上调,建立了一个调节轴.
结论:
- Circ_ZNF778_006在ESCC中充当瘤性circRNA,促进瘤进展和化学抵抗.
- 该circ_ZNF778_006/miR-18b-5p/HIF-1α轴代表了ESCC治疗的潜在治疗目标.
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