蒸发诱导的水力动力学在微生物群体中促进结合介导的等离子体转移
Chujin Ruan1,2, Josep Ramoneda2, Guowei Chen3
1College of Land Science and Technology, China Agricultural University, 100193, Beijing, China.
ISME communications
|November 8, 2023
概括
蒸发驱动水流,增加微生物等离子体的转移,尽管基因表达较低. 这项研究揭示了影响抗生素耐药性和微生物群落其他关键特征传播的物理力量.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物物理学的生物物理.
背景情况:
- 结合性等离子体赋予微生物群体抗生素耐药性和毒性等基本特征.
- 虽然已知等离子体生物学,但驱动其传播的物理和生态因素尚不清楚.
- 在不和环境中的微生物群体经历水蒸发和流体动力学.
研究的目的:
- 研究蒸发引起的水流对微生物细胞分布和等离子体结合的影响.
- 测试蒸发会影响细胞的空间动态和表面沉积,影响结合的假设.
- 探索蒸发,细胞接近和等离子体转移的效率之间的关系.
主要方法:
- 利用滴滴实验来模拟不和条件.
- 采用抗生素耐药性编码等离子体来追踪结合事件.
- 在蒸发条件下量化细胞分布,细胞间距离和结合率.
主要成果:
- 由蒸发引起的水流显著减少了细胞间的距离.
- 由于蒸发,等离子体结合的程度大大增加.
- 与结合相关的基因表达水平在蒸发下矛盾地较低.
结论:
- 蒸发提高了等离子体结合效率,这可能是由于细胞的接近度增加.
- 诸如蒸发之类的物理力量在等离子体传播的特征中起着至关重要的作用.
- 这些发现为微生物生态系统中抗生素耐药性和其他适应性特征的传播提供了新的视角.
相关概念视频
Mechanism of Conjugation
25
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
25
Conjugation
22
Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...
22
Transduction
21
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
21
Plasmids
21
Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
21
Horizontal Gene Transfer
19
Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...
19
Transformation
15
Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
15


