来自实验室环境的DNA吸收驱动了热爱者对中小成分的意想不到的适应
Benjamin Zeldes1, Anja Poehlein2, Surbhi Jain3
1Microbiology, Institute of Biological Sciences, University of Rostock, Rostock, Germany.
ISME communications
|November 8, 2023
概括
在实验室中,水平基因转移 (HGT) 意外地为Thermoanaerobacter kivui提供了新的特征,并改变了其基因组. 这强调了在涉及DNA吸收的微生物研究中需要仔细监测.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- DNA吸收是一种微生物适应的策略,通常在受控的实验室环境中进行研究.
- 微生物可以适应新的条件,但实验室条件可能会引入意想不到的遗传事件.
- 热甲氧杆菌kivui适应在一氧化碳 (CO) 上生长,表现出不寻常的表型.
研究的目的:
- 调查CO适应的T. kivui菌株中不寻常的表型的原因.
- 在实验室环境中识别水平基因转移 (HGT) 的来源和影响.
- 评估意外HGT事件的可复制性和可检测性.
主要方法:
- 在CO适应之前和之后对T. kivui进行比较基因组分析.
- 通过从Thermoanaerobacter sp.提供基因组DNA来进行HGT的实验复制. 菌株X514转变为T. kivui. 的.
- T. kivui菌株的表型特征,包括生长分析和维生素依赖.
主要成果:
- 来自Thermoanaerobacter sp.的一个水平基因转移 (HGT) 事件. 对T. kivui的X514菌株进行了鉴定.
- 转移的基因使T. kivui能够利用三糖,而被删除了一个本地胺生物合成操作子.
- 在实验室中,HGT是可复制的,并且由于宿主基因组的持久性和缺乏供体细胞,难以检测.
结论:
- 在实验室环境中,微生物之间可能发生意外的HGT,导致同时适应和遗传改变.
- 这种微妙的"基因组污染"形式需要仔细监测微生物表型和基因型.
- 对DNA吸收的自然能力需要谨慎预防和检测研究环境中意想不到的遗传交换.
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