相关实验视频

Updated: Jul 11, 2025

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions

Published on: June 14, 2016

拉动流量探针用于基于环境液体提取的高空间分辨率质谱成像,具有增强的灵敏度和稳定性

Zhihao Zhao¹, Zheng Long¹, Huabei Wang¹

¹College of Chemistry and Chemical Engineering, Central South University, Hunan, Changsha 410083, P. R. China.

Analytical chemistry

|November 8, 2023

概括

一个新的拉动流量探针通过提高生物组织中脂质分析的灵敏度,稳定性和空间分辨率来增强基于环境液体提取的质谱成像 (MSI).

科学领域:

分析化学分析化学
质谱仪成像成像质谱仪成像
生物医学工程生物医学工程

背景情况:

基于环境液体提取的质谱成像 (MSI) 提供直接分析,样本准备最少.
现有的集成探测器缺乏高空间分辨率,灵敏度和环境MSI的稳定性.
需要改进的采样探头来推进环境MSI应用.

研究的目的:

开发和评估一种新的集成探头,即拉动流量探头,用于基于环境液体提取的MSI.
在质量转移,灵敏度,稳定性和空间分辨率方面,比较拉动流量探针与单探针的性能.
为了证明拉出的流量探针在生物组织中的高分辨率脂质成像中的实用性.

主要方法:

使用同轴毛细血管制造一个拉动的流量探针.
调查质量转移动力学和计算流体动力学分析.
性能评估使用基于环境液体提取的脂肪的MSI在老鼠大脑和海马,和一个统一的墨水涂层稳定性评估.

主要成果:

与单探头相比,拉动流量探头表现出更高的提取效率和更低的波段扩散.
使用拉出的流量探针的MSI显示了2-5倍高的低丰度脂质信号,并检测到26种更多的脂质物种.
拉出的流量探测器显示稳定性显著改善 (RSD 18% vs 80%) 和更高的空间分辨率 (30-40 μm).

结论:

拉动流量探头是一种易于制造的采样探头,可显著提高基于环境液体提取的MSI的灵敏度,稳定性和空间分辨率.
这种新型的探测器在生物样本中促进了高分辨率的脂质成像,使其能够局部化到特定的细胞层.
拉出的流量探针对环境MSI的生物和临床研究应用具有宝贵的进步.

更多相关视频

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry

Published on: September 3, 2010

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

相关概念视频

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

High-Performance Liquid Chromatography: Types of Detectors

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...