阿司匹林与含胆固醇的膜以pH取决的方式相互作用
Michael Krmic1, Escarlin Perez1, Patrick Scollan1
1Department of Chemistry and Biochemistry, Iona University, 715 North Avenue, New Rochelle, New York 10801, United States.
Langmuir : the ACS journal of surfaces and colloids
|November 8, 2023
概括
阿司匹林增加了细胞膜的水透性,特别是在中性状态下. 这种效应受到胆固醇度和pH值的影响,影响生物膜,并可能解释长期使用阿司匹林引起的胃肠毒性.
科学领域:
- 生物物理学的生物物理.
- 膜生物物理学 膜生物物理学
- 药理学 药理学是指药理学的学科.
背景情况:
- 阿司匹林广泛用于预防心血管疾病,并与脂质膜相互作用.
- 像阿司匹林这样的非类固醇抗炎药物 (NSAID) 可以改变细胞膜的生物物理性质.
研究的目的:
- 为了研究阿司匹林对混合脂质模型双层中跨层层水透性的影响.
- 检查胆固醇度和pH值如何影响阿司匹林与脂质膜的相互作用.
主要方法:
- 使用液滴界面双层方法制备混合脂质模型双层 (POPC或DOPC具有不同胆固醇度).
- 在不同的pH条件下 (pH3和pH7) 评估跨层水的透性.
- 从差异扫描热度计,共聚焦拉曼显微光谱计和界面张力计的数据的整合.
主要成果:
- 阿司匹林以度依赖的方式增加了POPC双层的水透性,pH 3时的效果比pH 7时更大.
- 胆固醇调节了阿司匹林对水的透性的影响,在高胆固醇水平和pH 7时观察到的增强较小.
- 阿司匹林引起了显著的热,结构和界面干扰,表明膜流化和干扰作用.
结论:
- 阿司匹林使胆膜流化,既没有胆固醇,又富含胆固醇.
- 当药物处于中性状态时,阿司匹林的膜流化作用更加明显.
- 研究结果提供了阿司匹林对各种生物膜的影响的见解,这与理解NSAID诱导的胃肠道毒性有关.
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