通过下一代测序识别人类唾液中的习惯特异性细菌的识别
Shubham Yadav1, Vijay Tripathi2, Vaibhav Saran1
1Department of Forensic Science, Sam Higginbottom University of Agriculture, Technology And Sciences, Prayagraj, Uttar Pradesh, India.
Forensic science international
|November 8, 2023
概括
吸烟,饮酒和饮食等生活习惯显著改变了唾液微生物群. 下一代测序揭示了与这些习惯相关的独特微生物特征,影响了口腔健康.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 口腔健康 口腔健康
背景情况:
- 人的唾液微生物群是复杂的,受到各种因素的影响.
- 了解这些影响对于口腔健康至关重要.
- 下一代测序 (NGS) 提供了一种强大的方法来研究微生物群落.
研究的目的:
- 研究生活习惯 (吸烟,饮酒,素食饮食) 如何影响唾液微生物组的组成和多样性.
- 为了确定与每个习惯相关的特定微生物变化.
主要方法:
- 从具有不同习惯的个体收集唾液样本.
- 16S rRNA基因的V3-V4区域的DNA提取和放大.
- 下一代测序 (NGS) 和生物信息学分析用于微生物分析.
主要成果:
- 在不同的习惯中观察到不同的唾液微生物组概况.
- 吸烟者表现出更多的潜在致病性细菌.
- 饮酒改变了微生物多样性;纯素饮食与特定的细菌患病率有关.
结论:
- 生活习惯显然塑造了唾液微生物组.
- 习惯特定的微生物特征对口腔健康有影响.
- 基于NGS的唾液微生物组分析可以为个性化的健康策略提供信息.
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