异黄不调节miRNA分泌的模式:对神经发生的内分泌影响
Lizhu Tang1, Jing Li1, Chenyan Hu2
1Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Environment international
|November 8, 2023
概括
4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) 导致内分泌系统的干扰和神经毒性. 微RNAs (miRNAs) 调解了内分泌和神经系统之间的这种有毒交叉声,影响神经发生.
科学领域:
- 毒理学 毒理学 毒理学
- 内分泌学 在内分泌学.
- 神经科学是一个神经科学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 异硫酸是常见的防腐剂,健康问题越来越严重.
- 4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) 与内分泌干扰和神经毒性有关.
- 这些有毒效应之间的通讯途径尚未得到充分理解.
研究的目的:
- 研究微RNAs (miRNAs) 在调解DCOIT引起的内分泌干扰和神经毒性之间的交叉语音中的作用.
- 探索DCOIT如何影响内分泌细胞中的miRNA表达和分泌以及它们对神经元细胞的影响.
主要方法:
- H295R细胞被暴露在DCOIT中,以分析细胞内和细胞外的miRNA变化.
- 来自DCOIT治疗的H295R细胞的细胞外miRNA通过外体被分离出来.
- 用这些细胞外miRNA处理PC12神经细胞,以评估神经发生效应.
主要成果:
- DCOIT暴露改变了H295R细胞中的349个细胞内小RNA,与内分泌通路有关.
- 在细胞外介质中发现了66种差异表达的miRNA,与细胞内形状不同.
- 来自DCOIT压力细胞的细胞外miRNA促进了PC12细胞中神经元外生长和神经元分化.
结论:
- 微RNAs在DCOIT的系统毒理学中充当信号通讯器.
- 细胞外miRNAs调解毒性信息的传输,从内分泌干扰到神经毒性.
- miRNAs代表了一个潜在的表观遗传机制,是DCOIT双重毒性的基础.
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