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传感器的光遗传学:按需的光标签的基因素表观遗传学

Afanasii I Stepanov¹, Polina A Zhurlova², Alexandra A Shuvaeva³

¹Center for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Bolshoy Boulevard 30, 121205 Moscow, Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.

Biochemical and biophysical research communications

|November 8, 2023

概括

我们开发了一种新的光激活传感器,可在活细胞中可视化素H3 lysine-9三甲基化 (H3K9me3). 这种光遗传工具允许按需成像,最大限度地减少对自然表观遗传调节的干扰.

科学领域:

表观遗传学和分子生物学
光遗传学和活细胞成像学
染色体的动态染色体的动态

背景情况:

质子修饰对于染色质调节和细胞功能至关重要.
基因编码的光传感器可以动态监测活细胞中的表观遗传状态.
现有的传感器可能会因为长时间的染色质结合而干扰内源性表观遗传过程.

研究的目的:

开发一种光遗传传感器,可实时可视化素H3 lysine-9三甲基化 (H3K9me3).
通过控制传感器定位来最大限度地减少正常表观遗传调节的潜在干扰.
为了实现对H3K9me3丰富的染色质位点的按需可视化.

主要方法:

一个融合蛋白 (MPP8-LAMS) 的构造,包括一个核出口信号,一个远红色的光蛋白,一个H3K9me3结合域和一个光敏感域.
使用蓝光刺激来诱导传感器的核转位.
使用光显微镜对H3K9me3定位的活细胞成像.

主要成果:

在蓝光暴露时,MPP8-LAMS传感器证明了从细胞质到细胞核的高效转移.
传感器可以可视化活细胞中H3K9me3丰富的位置.
传感器的光激活性质使其与染色质的相互作用时间最小化,减少了潜在的生理影响.

结论:

开发的光遗传传感器为可视化活细胞中的H3K9me3修饰提供了一种新的方法.
根据需求激活传感器可以减少对内生表观遗传机制的干扰.
这项工作扩大了光遗传学用于先进的遗传编码传感器开发的应用.

关键词:

根据ASLOV2的规定.表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.H3K9me3是什么意思 H3K9me3是什么意思基因组胺基因的修改活细胞成像成像技术核进口核进口核进口核进口视觉遗传学视觉遗传学

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