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Updated: Jul 11, 2025

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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核糖体蛋白S24在Macrobrachium nipponense的卵巢发育中的分子和功能特征

Hongxia Jiang¹, Xiao Li¹, Yizheng Li¹

¹Engineering Lab of Henan Province for Aquatic Animal Disease Control, Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation, College of Fisheries, Henan Normal University, Xinxiang, Henan 453007, China.

International journal of biological macromolecules

|November 8, 2023

概括

在 (Macrobrachium nipponense) 中的RPS24基因促进卵巢发育. 这种核糖体蛋白刺激卵成熟的关键基因,为水产养殖挑战提供解决方案.

科学领域:

水产养殖是水产养殖的一种方式.
分子生物学分子生物学
发展生物学发展生物学

背景情况:

核糖体蛋白 (RPs) 具有外核糖体功能,包括调节各种生物体的卵巢发育.
快速的卵巢成熟是Macrobrachium nipponense水产养殖的一个重大挑战.

研究的目的:

为了识别和表征RPS24基因 (MnRPS24) 在Macrobrachium nipponense.se中.
调查MnRPS24在卵巢发育和小胞形成中的作用.

主要方法:

在不同的生长阶段和组织中进行基因识别和表达分析.
MnRPS24蛋白的亚细胞局部化.
在体外实验中使用重组MnRPS24蛋白来评估基因表达变化.
对MnRPS24的基因沉默,以评估其对卵巢发育和细胞生成的影响.

主要成果:

成年女性,卵巢和I期卵巢的MnRPS24表达最高.
MnRPS24蛋白位于卵细胞的细胞质和周围的卵泡细胞中.
再组合的MnRPS24上调调节了参与生 (MnVg,MnVgr) 和细胞循环进展 (MnCyclin B,MnCdc2) 的基因,同时降低了MnWee1.1.
抑制MnRPS24基因沉默抑制了小细胞发生和延迟了卵巢发育.

结论:

MnRPS24在促进卵巢发育方面发挥着至关重要的作用,在Macrobrachium nipponense.
MnRPS24作为活细胞生成和卵细胞成熟的刺激剂.
研究结果表明,MnRPS24是改善水产养殖实践的潜在目标.

关键词:

马克罗布拉基乌尼波尼尼尼斯卵巢发育卵巢发育卵巢爆炸化器的潜伏期再组合蛋白质是一种重组蛋白质.核糖体蛋白质S24 核糖体蛋白质S24 小的干扰RNA可以干扰RNA.

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