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细胞膜内膜炎 sp. 细胞膜内膜炎 sp. nov.:一种从子宫内膜微生物群中分离出来的新型细菌
Linda Abou Chacra1,2, Marion Bonnet1,2, Mégane Heredia1,2
1Aix-Marseille Université, IRD, AP-HM, SSA, VITROME, Marseille, France.
Archives of microbiology
|November 8, 2023
概括
一个新型的细菌物种,细胞膜内膜菌 (Cellulomonas endometrii sp. 11月,从子宫内膜活检中确定. 这一发现扩大了已知的Cellulomonadaceae家族和Actinomycetota族群中的多样性.
科学领域:
- 微生物学 微生物学
- 细菌的分类学 细菌的分类学
- 基因组学就是基因组学.
背景情况:
- 细菌鉴定对于了解传染病至关重要.
- 马尔迪-托夫质谱法是一种常见的识别方法.
- 一些细菌隔离物需要先进的技术来准确分类.
研究的目的:
- 识别和描述从子宫内膜活检中分离的新型细菌.
- 确定新细菌物种的分类学分类.
- 提出一种新的物种名称和类型菌株.
主要方法:
- 16S rRNA基因测序和BLASTn分析用于初始识别.
- 现型特征包括Gram染色,运动性和子形成.
- 基因组测序和G+C含量的分析.
- 细胞壁脂肪酸分析.
- 使用数字DNA-DNA杂交 (dDDH) 和正统平均核酸标识 (OrthoANI) 的基因组比较.
主要成果:
- 隔离物显示出与细胞原虫 (98.85%) 和巴基斯坦细胞原虫 (98.45%) 的最高序列一致性.
- 现型检测显示了一种可选的无氧,阳性,运动性,棒状细菌.
- 基因组大小为4.25 Mbp,G+C含量为74.8 mol%.
- dDDH和OrthoANI值低于既定物种值,支持新的物种名称.
- 12甲基四甲基酸是最丰富的细胞壁脂肪酸 (36%).
结论:
- 这种分离物体代表了Cellulomonadaceae家族和Actinomycetota phylum中的新物种.
- 这种新物种的拟议名称是Cellulomonas endometrii sp. 在新的一年里.
- 类型菌株是马赛-Q7820T (CSUR Q7820 = CECT 30716) 的.
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