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从临床样本中分离的ESBL产生肠杆菌和无症状便载体之间的显著关系

Shadi Aghamohammad¹, Fereshteh Shahcheraghi²

¹Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.

BMC infectious diseases

|November 8, 2023

概括

产生扩展谱β-乳糖酶 (ESBL) 的Enterobacteriaceae菌株在便载体和患者中表现出类似的分子特征. 这表明ESBL-KP可以广泛殖民,强调需要便载体监测以减少水库.

科学领域:

微生物学微生物学
分子生物学分子生物学
传染性疾病传染性疾病

背景情况:

从便载体和患者中对肠道细菌中的扩展谱β-乳糖酶 (ESBL) 的研究至关重要.
了解携带者与患者中的耐药细菌特征,可以揭示隐藏的储存库并放大抗菌素耐药性.

研究的目的:

为了比较来自临床和便载体来源的ESBL产生的Enterobacteriaceae菌株的抗微生物耐药性模式和分子特征.
评估ESBL产生的Klebsiella肺炎和大肠杆菌的克隆相关性和分子特征.

主要方法:

收集了142个临床样本和120个直肠拭子.
使用双盘协同测试用于ESBL查和PCR用于ESBL基因检测.
评估生物膜形成,毒性基因,K. pneumoniae的MLVA,以及E. coli的类组类型.

主要成果:

发现了大肠杆菌 (67.6%) 和肺炎 (32.4%). 在 blaCTXM-15 基因中, blaCTXM-15 基因是普遍存在的 (89.4%).
基因组B2在ESBL-E. coli (56.2%) 中最常见. 在K. pneumoniae分离物中,Ompk35和FimH的表达率很高.
分子类型测定揭示了临床和载体分离物之间的相关性.

结论:

分子特征和克隆相关性表明ESBL-KP菌株可以在不同的环境中以类似的方式殖民,这表明广泛的社区和医院分布.
实施控制协议,包括便载体监测,可以减少无症状储存和患者感染率.

关键词:

临床来源临床来源克隆相关性克隆相关性它们是Enterobacteriaceae的细菌.便运输车便运输车

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