使用下一代测序揭示腹腹的病因学
Merili Saar1,2, Risto Vaikjärv3, Ülle Parm1,4
1Department of Microbiology, Institute of Biomedicine and Translational Medicine, Faculty of Medicine, University of Tartu, Ravila 19, Tartu, 50411, Estonia.
Annals of clinical microbiology and antimicrobials
|November 8, 2023
概括
下一代测序确定了Streptococcus pyogenes,Fusobacterium necrophorum和Fusobacterium nucleatum作为腹 (PTA) 中的关键细菌. 样品比桃体活检更具代表性,用于诊断PTA感染.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 腹腹 (PTA) 是一种严重的深感染,细菌原因不明.
- 了解涉及的特定细菌对于有效治疗至关重要.
研究的目的:
- 使用下一代测序 (NGS) 识别PTA的细菌病因.
- 为了比较桃体活检和PTA患者的样本中的微生物群落.
主要方法:
- 来自91名PTA患者的桃体活检和样的分析.
- 应用下一代测序 (NGS) 进行全面的细菌鉴定.
主要成果:
- 确定了400多种属和800多种物种,其中Streptococcus pyogenes,Fusobacterium necrophorum和Fusobacterium nucleatum是最丰富的.
- S. pyogenes经常占主导地位,但F. nucleatum和F. necrophorum也经常主导样本.
- 在和桃体活检样本之间的微生物组成中没有发现显著差异.
结论:
- 杆菌 (Streptococcus pyogenes),Fusobacterium necrophorum和Fusobacterium nucleatum可能是PTA的主要致病剂.
- 其他细菌,如Streptococcus anginosus和厌氧菌也可能导致PTA.
- 样本对PTA的微生物学检查比桃体组织更具代表性,有助于患者管理.
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