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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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建立细胞计量实验室的准则

Anna C Belkina1, Caroline E Roe2, Vera A Tang3

  • 1Flow Cytometry Core Facility, School of Medicine, Boston University, Boston, Massachusetts, USA.

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概括

本指南为建立和管理流细胞计共享资源实验室 (SRL) 提供了最佳实践. 它涵盖了卓越的运营,技术和人员,以满足科学界的需求.

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Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
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Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
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Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
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科学领域:

  • 生物技术是生物技术.
  • 实验室管理 实验室管理
  • 科学仪器仪表科学仪器仪表

背景情况:

  • 流细胞计共享资源实验室 (SRLs) 对于推动生物研究至关重要.
  • 有效的管理和卓越的运营是最大限度地利用这些专业设施的关键.
  • 以前的指南提供了一个基础,但进步需要更新最佳实践.

研究的目的:

  • 为建立和维护流细胞计SRL提供全面指导.
  • 概述操作卓越和满足科学界需求的优先目标.
  • 详细介绍相关技术,软件,运营要求和管理策略.

主要方法:

  • 来自国际细胞计量进步协会 (ISAC) 共享资源实验室 (SRL) 新兴领导者计划的专业知识汇编.
  • 审查细胞计量技术和管理实践的最新进展.
  • 纳入最佳实践的住房,运行和支持的流细胞计设备.

主要成果:

  • 概述了在流细胞计SRL中优先考虑运营卓越的关键目标.
  • 提供了有关可用的细胞计量技术和相关软件的信息.
  • 详细介绍了员工,管理和运营要求的最佳实践.

结论:

  • 本资源有助于建立新的流量细胞计SRL,重新利用现有空间,或将流量细胞计集成到单个实验室.
  • 坚持最佳实践确保了高效的运作和对科学界的持续支持.
  • 该指南强调了关于位置,空间,资源和支持的仔细规划的重要性,以确保SRL的成功运营.