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Updated: Jul 11, 2025

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高保真性大多样性单克隆哺乳动物细胞库通过细胞周期停止的复合酶介导的磁带交换

Chuan Chen1, Zening Wang1,2, Minhyo Kang1

  • 1Department of Chemical and Environmental Engineering, University of California Riverside, Riverside, CA 92521, USA.

Nucleic acids research
|November 9, 2023
PubMed
概括

一种新的方法,细胞循环停止的复合酶介导磁带交换 (aRMCE),使哺乳动物细胞中高质量的组合图书馆成为可能. 这促进了基因工程的研究和治疗开发.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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