比较的叶绿体基因组提供了对Datureae植物进化和物种识别的见解
He Su1,2, Xiaoxia Ding1, Baosheng Liao1
1The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China.
Frontiers in plant science
|November 9, 2023
概括
这项研究分析了13个Datureae物种的叶绿体基因组,揭示了对分类学和原生学至关重要的保存但可变的区域. 开发了一种新的特定物种标记,用于识别Datura品种.
科学领域:
- 植物基因组学 植物基因组学
- 分子进化的分子进化.
- 生物信息学是一种生物信息学.
背景情况:
- 血管苗中的叶绿体基因组通常是保存的,但表现出种际变异.
- 包含装饰植物和药用植物的Datureae部落是研究这些变异的理想模型.
研究的目的:
- 分析和比较13个Datureae物种的叶绿体基因组.
- 为了解决Datureae部落内的家族遗传关系.
- 开发一种特定物种的标记物来识别Datura.
主要方法:
- 对13个Datureae质细胞基因组进行比较分析,并补充了NCBI的9个现有基因组.
- 基因组大小,基因含量和重复分析 (SSR,长,并列重复).
- 使用塑体基因组进行遗传学分析,并开发一种特定物种标记物.
主要成果:
- 叶绿体基因组大小在154,686至155,979个基因组之间.
- 鉴定了每种128-132个基因 (83-87个蛋白质编码,37个tRNA,8个rRNA).
- 遗传学分析证实了Datura,Brugmansia和Trompettia的单性;成功开发了D. stramonium及其品种的特定物种标志物.
- 31个基因,包括ATP,光系统,核糖体和NAD(P) H脱酶的基因,显示出积极选择的迹象,表明适应.
- 达图雷亚的多样化始于奥利戈纪晚期.
结论:
- 分析的叶绿体基因组为Datureae的分类学,遗传学和进化研究提供了宝贵的遗传资源.
- 特定基因的积极选择表明适应不同环境.
- 开发的标记物是有效的区分D.斯特拉和D.斯特拉变种. 无力. 无力. 无力.
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