评价氧化铁纳米颗粒的肝毒性与毒性作用:进一步了解剂量依赖的氧化作用
Basma Emad Aboulhoda1, Doaa Abdullah Othman1, Laila A Rashed2
1Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Egypt.
氧化铁纳米颗粒 (IONPs) 在老鼠中引起了剂量依赖的肝脏和脏损伤. 较高的IONP剂量显著增加肝酶和氧化应激标志物,表明毒性.
科学领域:
- 毒理学 毒理学 毒理学
- 纳米技术 纳米技术
- 生物化学 生物化学
背景情况:
- 氧化铁纳米粒子 (IONPs) 越来越多地用于生物医学应用.
- 了解它们的潜在毒性对于安全应用至关重要.
- 之前的研究表明,IONP对生物系统的影响各不相同.
研究的目的:
- 研究IONPs对大鼠肝脏和脏的剂量依赖性毒性影响.
- 评估不同IONP剂量引起的生化和组织学变化.
- 为了比较IONP对肝脏和脏组织的影响差异.
主要方法:
- 在28天的时间里,小鼠接受了三种不同剂量的IONPs (30,300,1000 mg/kg/天).
- 测量了肝酶 (AST,ALT,ALP),尿素,肌,MDA和铁的血清水平.
- 评估了抗氧化酶 (SOD,GPx) 的活性.
- 组织病理学检查包括原沉积,α-SMA,PAS反应和iNOS表达.
主要成果:
- 在中高剂量组中,AST,ALT和ALP显著升高.
- 在中高剂量组中增加尿素,肌素和MDA水平.
- 在高剂量组中,SOD和GPx活性降低.
- 血清铁水平的剂量依赖性增加.
- 在高剂量时,与脏相比,肝脏中的原沉积和α-SMA表达更大.
- 肝脏和脏之间对PAS和iNOS表达的不同影响.
结论:
- 在老鼠肝脏和脏中,IONPs表现出剂量依赖的毒性.
- 较高的IONP剂量会诱导严重的肝脏和脏损伤,由生化和组织学变化证明.
- 肝脏似乎比脏更容易受到IONP诱导的纤维和炎症变化.
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