人体红血细胞释放出不同大小和蛋白质组成的微粒,改变中性粒细胞细胞形成
Getulio Pereira de Oliveira Junior1,2, Joshua A Welsh3, Brandy Pinckney4
1Division of Allergy and Inflammation, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Journal of extracellular biology
|November 9, 2023
概括
人体红血细胞 (RBC) 产生独特的微囊 (RBC-MVs),与中性粒细胞相互作用,影响其细胞形成. 这项研究揭示了对红细胞-MV生物发生和循环中的功能的新见解.
科学领域:
- 细胞生物学 细胞生物学
- 血液学 血液学 血液学
- 生物化学 生物化学
背景情况:
- 细胞外囊泡 (EVs) 中介细胞间的通信.
- 红血细胞 (RBC) 很丰富,并释放微粒细胞 (RBC-MVs).
- 红细胞-MVs的体内命运和功能在很大程度上是未知的.
研究的目的:
- 研究红细胞-大脑小细胞的生物发生,特征和功能影响.
- 探索红细胞-MV与其他循环细胞,特别是中性粒细胞的相互作用.
主要方法:
- 使用大小排除色谱 (SEC) 和密度梯度电泳 (DEG) 隔离红细胞-MVs.
- 通过流细胞计,多角度雷利散射 (MRPS) 和免疫传递电子显微镜 (TEM) 进行表征.
- 蛋白质组分析和冷电子显微镜用于相互作用研究.
主要成果:
- 人体红细胞产生两种不同类型的红细胞-MV,它们的尺寸,密度和蛋白质成分各不相同.
- 红细胞-大小细胞富含参与离子运输和囊泡贩运的蛋白质.
- 低温EM显示,红细胞-小微小细胞通过融合或内化与中性粒细胞相互作用.
- 红细胞-MVs抑制大肠杆菌的中性细胞化,而不会影响细胞存活.
结论:
- 红细胞-MVs表现出复杂的生物发生和独特的功能性质.
- 红细胞-MV细胞在调节循环中的中性粒细胞功能的过程中发挥作用.
- 这项研究为红细胞衍生的细胞外囊泡及其生理相关性提供了新的见解.
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