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纤维细胞改变皮肤ECM衍生的水凝的物理性质,以创建一个亲血管性微环境

Meng Zhang^1,2, Fenghua Zhao^2,3, Xue Zhang^1,2

¹University of Groningen, University Medical Centre Groningen, Department of Pathology and Medical Biology, Hanzeplein 1 (EA11), 9713, GZ Groningen, the Netherlands.

Materials today. Bio

|November 9, 2023

概括

纤维细胞 (MRC-5) 显著改变细胞外基质 (ECM) 微环境,增强血管化. 纤维细胞对ECM的这种动态重塑促进了皮肤衍生水凝中的内皮细胞 (EC) 的生长.

科学领域:

生物材料科学生物材料科学
细胞生物学细胞生物学
组织工程是组织工程.

背景情况:

内皮细胞 (ECs) 对于血管化至关重要.
细胞外矩阵 (ECM) 的微环境影响了EC的行为.
纤维细胞在组织重塑和血管化中发挥作用.

研究的目的:

研究MRC-5纤维细胞在体外血管化过程中对内皮细胞 (ECs) ECM微环境的影响.
分析由于纤维细胞的存在而导致的水凝特性和ECM组成的变化.
了解纤维细胞在通过ECM重塑促进血管化的作用.

主要方法:

人体皮肤微血管内皮细胞 (HMEC) 和人体肺微血管内皮细胞 (HPMEC) 与皮肤衍生ECM水凝中的MRC-5纤维细胞共同培养.
评估水凝的机械性能 (刚性).
分析ECM纤维直径,多孔性,原重塑和纤维素-1和纤维菌素的沉积.

主要成果:

MRC-5纤维细胞增加了水凝的刚性,纤维直径和多孔性.
在ECM水凝中观察到显著的原纤维重塑.
与MRC-5纤维细胞共同培养中发生了纤维素-1和纤维素蛋白沉积的增加.
在纤维细胞的存在下观察到增强的血管化.

结论:

MRC-5纤维细胞动态重塑ECM的微环境.
纤维细胞介导的ECM重塑促进了血管化.
这项研究强调了纤维细胞-ECM相互作用在工程血管组织中的重要性.

关键词:

生物力学生物力学原蛋白是一种原蛋白.在ECM中使用水凝.内皮细胞是内皮细胞.细胞外矩阵是细胞外矩阵.纤维纤维素的生长方式血管化血管化

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