用光染料和膨胀显微镜对人类亚细胞区的差异标记
Thomas M D Sheard1, Tayla B Shakespeare1, Rajpinder S Seehra1
1School of Biosciences, Faculty of Science, University of Sheffield, Sheffield S10 2TN, UK. t.sheard@sheffield.ac.uk.
Nanoscale
|November 9, 2023
概括
光染料 Ester 有效地染色细胞结构,揭示纳米尺度的细节,当使用扩展显微镜 (ExM). 它们的标签模式取决于染料特性和应用时间,提供多功能成像应用.
科学领域:
- 细胞生物学 细胞生物学
- 显微镜的使用方法
- 生物化学 生物化学
背景情况:
- 芳香光染料的氨基反应性 Ester 越来越多地被用作成像探针.
- 这些探针对于染色细胞和组织架构非常有价值.
研究的目的:
- 描述14种光染料 Ester 的染色模式.
- 为了评估它们的实用性与扩展显微镜 (ExM) 结合用于纳米尺度成像.
主要方法:
- 用14种光染料乙物种对人类HeLa细胞进行染色.
- 使用超分辨率膨胀显微镜 (ExM) 与可膨胀的烯胺水凝.
- 基于染料特性,应用时间和之间相互作用的染色模式的分析.
主要成果:
- 光 Ester 揭示了纳米级的细胞特征,如膜结合的隔间和核密度与 ExM.
- 观察到不同的标签模式,与染料的生物化学性质相关.
- 在ExM协议中,染色密度和特异性因应用时间而异.
- 间相互作用为标签模式带来了进一步的复杂性.
结论:
- 光 Ester 是可视化细胞蛋白质组的工具包中的一个有价值的补充.
- 它们可以独立地用于形态学,也可以作为超结构性背景的反污染剂.
- 考虑它们的使用包括染料特性,应用时间和潜在的相互作用.
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