在植物组织中测试效应器细胞对细胞的移动性,确定了高移动性和等离子体的间接操纵
Mina Ohtsu1, Joanna Jennings1, Matthew Johnston1
1Cell and Developmental Biology, John Innes Centre, Norwich Research Park, U.K.
Molecular plant-microbe interactions : MPMI
|November 9, 2023
概括
研究人员确定了真菌病原体效应物如何在植物细胞之间移动. 他们发现,一些效应剂增强了等离子体的功能,改善了细胞间的通信和了解植物病原体相互作用.
科学领域:
- 植物病理学 植物病理学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 等离子体对植物细胞通信和资源交换至关重要.
- 植物病原体利用等离子体进行入侵,但效应器细胞间移动的好处尚不清楚.
研究的目的:
- 开发和应用定量实时成像方法,以识别和表征由真菌病原体*Colletotrichum higginsianum*的细胞对细胞移动效应体.
- 研究移动效应器对等离子体功能和细胞间连接性的影响.
主要方法:
- 根据表达,分泌信号和定位,预测了候选*C. higginsianum*效应者.
- 使用定量实时成像屏幕测试核细胞效应因子的细胞间移动性.
- 测量了已识别的高移动效应器对等离子体功能的影响.
主要成果:
- 14个候选效应因子被确定为细胞间移动的.
- 三个效应器表现出"超移动性",超过它们的大小所预期的运动.
- 两个超移动效应器增加了无直接关联的等离子体输送能力.
结论:
- 开发的方法允许定量分析效应体细胞对细胞的移动性及其对等离子体的影响.
- 超移动效应器可以增强等离子体的功能,这表明一种病原体操纵植物细胞的机制.
- 这项工作提供了对效应器策略和植物细胞间通信的见解.
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