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相关概念视频

Regulated Protein Degradation02:58

Regulated Protein Degradation

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It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
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Inhibition of Cdk Activity02:34

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The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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Positive Regulator Molecules02:39

Positive Regulator Molecules

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Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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Negative Regulator Molecules01:23

Negative Regulator Molecules

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Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
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Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
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相关实验视频

Updated: Jul 11, 2025

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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多个与库林相关的E3酶调节了环林D1蛋白的稳定性.

Ke Lu1, Ming Zhang2, Guizheng Wei1

  • 1Research Center for Computer-aided Drug Discovery, Chinese Academy of Sciences, Shenzhen, China.

eLife
|November 9, 2023
PubMed
概括

新的E3酶,Keap1,DDB2和WSB2,通过调节其降解来控制cyclin D1的稳定性和细胞循环的进展. 这一发现为与癌症相关的细胞循环调节提供了新的见解.

关键词:
DDB2 DDB2 在线播放凯普1号码是什么意思一个Rbx1一个Rbx1这是WSB2的WSB2.细胞生物学 细胞生物学与库林相关的E3结合酶环 D1 D1 的时间.人类 人类 人类 人类 人类 人类 人类

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相关实验视频

Last Updated: Jul 11, 2025

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Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
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科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 在瘤学瘤学.

背景情况:

  • 环素D1是细胞周期G1/S过渡的关键调节剂,在癌症中经常被上调调节.
  • 它的稳定性主要受到基化/蛋白酶体降解的调节,受酸化的影响.
  • 已知的调节剂包括与库林相关的F-盒E3链酶,但可能存在其他.

研究的目的:

  • 确定参与调节环林D1蛋白稳定性的新型林相关E3酶.
  • 研究这些E3酶在细胞周期控制和增殖中的作用.

主要方法:

  • 选了一个siRNA库,针对154个联酶子单元 (F-box,SOCS,BTB,DDB蛋白).
  • 评估了E3酶与环林D1.1的相互作用.
  • 分析了环林D1的泛化,蛋白酶体降解和酸化依赖的调节.
  • 对细胞周期进展和增殖的评估影响.

主要成果:

  • 确定Keap1,DDB2和WSB2是新型的与库林相关的E3酶,调节环林D1.
  • 证明这些E3酶与环林D1相互作用,以酸化依赖的方式促进其泛化和蛋白酶体降解.
  • 表明这些E3链酶通过循环D1稳定性影响细胞周期进展和增殖.

结论:

  • 多个与库林相关的E3酶,包括Keap1,DDB2和WSB2,在调节环林D1蛋白稳定性方面发挥着重要作用.
  • 这些发现扩大了我们对循环D1调节及其对细胞循环控制和癌症的影响的理解.