高通量液体染色学分析使用1秒周期时间的细分流量注入器
Devin M Makey1, Roger C Diehl2, Yue Xin1
1Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
Analytical chemistry
|November 9, 2023
概括
滴滴微流体学使高通量选 (HTS) 的液体染色学 (LC) 更快. 这种方法克服了自动采样器的速度限制,允许快速分析样本,用于药物发现和其他应用.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
背景情况:
- 高通量查 (HTS) 在药物发现和其他领域至关重要.
- 液体染色学 (LC) 在HTS中至关重要,但通常受到缓慢分离时间的限制.
- 现有的自动采样器限制了HTS工作流中的LC分离速度.
研究的目的:
- 为了克服高通量LC的自动采样器速度的局限性.
- 为了更快地引入样品,将液滴微流体与液体流体相结合.
- 为了证明这种方法在HTS应用中的实用性.
主要方法:
- 利用滴滴微流体进行样本引入,创建空气分段样本列车.
- 采用了一种新型的LC注入,通过样本循环填充来启动.
- 在高流速 (5毫升/分钟) 时使用了短,包装的LC柱 (2.1毫米×5毫米) 带有表面多孔粒子.
- 包含洗液滴,以最大限度地减少分析物携带.
主要成果:
- 对于3个组件的样本分离每次实现1秒.
- 能够在1.6分钟内对96个井板进行分析,RSD<2%.
- 通过使用洗滴来证明最小化了分析物携带.
- 与常规LC方法相关的结果在细胞染色体P450抑制屏幕中.
结论:
- 滴滴微流体学有效地克服了HTS.中的自动采样器速度限制.
- 这种细分流量注入方法使得快速,高通量LC分析.
- 该方法显示了彻底改变HTS工作流程的巨大潜力.
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