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候选基因中的多态性对子尸体和肉质特征的影响
Hosam M Safaa1,2, Mohamed Ragab3,4, Marwa Ahmed5
1Department of Biology, College of Science, University of Bisha, Bishah, Saudi Arabia.
研究子生长激素 (GH) 和其他基因中的单核酸多态 (SNPs) 发现了与肉质和尸体特征的显著关联. 这些发现表明,在子繁殖计划中,有潜在的标记器辅助选择.
科学领域:
- 动物遗传学动物遗传学
- 分子生物学分子生物学
- 畜牧科学 畜牧科学 畜牧科学
背景情况:
- 候选基因方法对于理解基因特征关联和在牲畜中推进标记器辅助选择至关重要.
- 识别与肉质和尸体特征相关的遗传变异对于有效的子繁殖至关重要.
研究的目的:
- 研究四个候选基因中的多态性:生长激素 (GH),胰岛素样生长因子-II (IGF-II),脂肪质量和与肥胖相关的 (FTO) 和胰岛素受体基质-1 (IRS-1).
- 确定这些单核酸多态 (SNP) 与新西兰白和巴拉迪子的尸体和肉质特征的关联.
主要方法:
- 使用限制片段长度多态聚合酶连锁反应 (RFLP-PCR) 的四个特定SNP的基因定型.
- 在两种子品种中,分析识别的基因型与各种尸体和肉质量参数之间的关联.
主要成果:
- 异合体基因型是最常见的,除了巴拉迪子中的FTO SNP.
- GH基因型显著影响了肉的轻度和后部的重量.
- IGF-II SNPs影响了屠宰,热尸体,商业尸体和后部重量.
- FTO SNPs与损失和肌肉内脂肪体重有关.
- 美国国税局-1 SNPs显示与滴滴损失和肌肉内脂肪有显著的关联.
- 观察到IGF-II对损失和肌肉内脂肪的品种特异性影响.
结论:
- 研究的GH,IGF-II,FTO和IRS-1基因的突变可能作为子选择的遗传标记物有用.
- 建议进行进一步的研究,以确定与体和肉质特征相关的额外变异,以改进子繁殖策略.
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