由Citrus tristeza病毒引起的茎与增加的花闭塞有关
Maryam Khalilzadeh1, Chun-Yi Lin2, Chunxia Wang2
1Department of Plant Pathology, University of Florida, Gainesville, FL, USA; Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
Virology
|November 9, 2023
概括
花悲伤病毒 (CTV) 通过扰乱花发育引起茎病 (SP). 在受感染的类树中,和花蛋白积累的增加与SP症状的严重程度相关,表明花封闭.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 植物生理学 植物生理学
背景情况:
- 果中由Citrus tristeza病毒 (CTV) 引起的茎病 (SP) 破坏了花和树皮的发育.
- 类植物中SP的一个关键症状是花再生,这表明受感染的花细胞的功能减少.
研究的目的:
- 调查SP症状严重程度与感染CTV的类巨型植物中花细胞封闭之间的关系.
- 分析与不同CTV突变体和SP表型相关的和叶膜蛋白 (PP) 的积累.
主要方法:
- 分析和花蛋白 (PP) 在类巨树中的积累.
- 感染各种CTV突变 (CTVΔp13,CTVΔp33),全长CTV和健康对照.
- 对PHLOEM-PROTEIN 2和BETA-1,3-GLUCANASE的基因表达分析.
主要成果:
- CTV感染导致质和PP积聚在体中的增加.
- 在SP症状严重程度和和PP水平之间观察到正相关性.
- 增加的PHLOEM-PROTEIN 2和减少的BETA-1,3-GLUCANASE基因表达伴随着更严重的SP症状.
结论:
- 在类植物中,茎插入症状的发展与增加的花封闭有关.
- 和膜蛋白积累是CTV诱导的膜功能障碍和SP严重性的关键指标.
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