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高标位 (>100 g/L) 乙醇生产从预处理的玉米炉水解由改性酵母菌株

Rui Zhao¹, Hongshen Li², Qi Li¹

¹Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China.

Bioresource technology

|November 9, 2023

概括

开发高效的纤维素乙醇生产需要有效的纤维素预处理和利用树脂素的酵母菌株. 这项研究设计了一种酵母菌株,DsnR4,从玉米炉水解剂中显示出高醇产量.

科学领域:

生物技术是生物技术.
生物化学工程生物化学工程
可再生能源可再生能源是可再生能源.

背景情况:

纤维素乙醇的生产依赖于高效的纤维素预处理和糖利用.
性煮有效地去除红素,并且需要工程酵母菌株来进行西洛斯发酵.

研究的目的:

开发一种可靠的纤维素预处理方法,用于混合糖提取.
设计高效的酵母菌株,用于在纤维素乙醇生产中利用氧化糖.

主要方法:

制的化学和特征分析,以去除木质素.
来自WXY70的酵母菌株 (ΔsnR4) 的工程,以提高糖的利用率.
在不同固体负载 (10%和高固体) 的玉米炉水解剂中对酵母性能的评估.
应用一个结合的NaOH球磨砂预处理策略.

主要成果:

性 significantly removed lignin from lignocellulose crops. 性显著地去除了葡萄素从葡萄素纤维素作物中的葡萄素. 性显著地去除了葡萄素从葡萄素纤维素作物中的葡萄素.
在15%的固体负载下,玉米炉水解剂的混合糖产量最高.
工程酵母菌株 ΔsnR4 在10%的固体中产生了高乙醇产量 (0.485 g/g总糖).
在高固体负荷下,通过NaOH球磨加工预处理,DsnR4在36小时内达到110.9g/L的最大乙醇标位 (92.9%的理论产量).

结论:

工程酵母菌株 ΔsnR4 与高固体负载的 NaOH 球型削预处理高度兼容.
ΔsnR4显示出工业纤维素乙醇生产的巨大潜力,因为它具有很高的乙醇产量和标位.
这项研究推进了用于可持续生物燃料开发的纤维纤维素值.

关键词:

性预处理是一种性预处理.滚球磨砂机的使用方法纤维素乙醇是一种纤维素乙醇.基纤维素化酸.这种植物是Saccharomyces cerevisiae.

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