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Updated: Jul 11, 2025

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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通过自我诱导的细胞内二硫化物配对来简化强大的异蛋白复合体的构建

Hyunji Kim1, Iji Yang1, Sung In Lim1

  • 1Department of Chemical Engineering, Pukyong National University, Yongso-ro 45, Nam-gu, Busan, Republic of Korea.

International journal of biological macromolecules
|November 9, 2023
PubMed
概括

研究人员为甲基结合域蛋白2 (MBD2) 和p66α设计了新的融合标签,以创建稳定的蛋白质复合体. 这种方法确保异体复合物即使在稀释时也保持完整,克服了以前的限制.

关键词:
卷曲卷曲的卷曲卷曲的卷曲.双硫化物键是一种二硫化物键.自动组装自动组装

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