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跨境的milRNAs:在Trichoderma breve T069的过程中不可或缺的分子 Botrytis cinerea的真菌寄生虫

Zhen Liu¹, Yuejiao Li¹, Jumei Hou¹

¹Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests (Hainan University), Ministry of Education, Haikou 570228, China.

Pesticide biochemistry and physiology

|November 9, 2023

概括

这项研究揭示了微RNA类小RNA (milRNAs) 是Trichoderma对致病性真菌B. cinerea.生物控制的关键. 这些milRNAs向重要的基因,为真菌疾病管理提供了新的策略.

科学领域:

菌类学菌类学是指菌类学.
植物病理学植物病理学
分子生物学分子生物学

背景情况:

越来越多的真菌微RNA类小RNA (milRNAs) 已被确定,但它们在生物控制真菌中的作用,特别是在真菌寄生虫期间,尚未得到充分研究.
了解寄生虫的milRNA功能可以揭示植物病原体的生物控制的新机制.

研究的目的:

在Botrytis cinerea的真菌寄生期间,在Trichoderma breve T069中对milRNAs进行分析.
为了识别特定的milRNAs及其参与真菌寄生虫相互作用的目标基因.
探索这些milRNAs作为对抗真菌病原体的生物控制剂的潜力.

主要方法:

构建了T. breve T069的GFP光菌株,以定义关键的真菌寄生虫病阶段 (早期,中期,晚期).
在关键相互作用期间使用高通量测序来识别T. breve T069和B. cinerea的小RNA (sRNA).
利用生物信息学来预测B. cinerea中的milRNA目标,专注于跨境的milRNAs,仅在T. breve中表达T069.

主要成果:

定义的真菌寄生病阶段:早期 (12小时,状平行),中期 (36小时,包裹) 和晚期 (72小时,退化).
在不同阶段的T. breve T069中确定了45个和73个潜在的milRNA目标.
发现了来自T. breve T069的14个跨境milRNA,针对B. cinerea中的41个基因,包括对真菌生长和致病性至关重要的基因 (例如Rim15,PKS3,Bcnrps6,奇合成酶7).

结论:

类似于微RNA的小RNA在B. cinerea.的Trichoderma介导的真菌寄生虫中发挥着重要作用.
来自生物控制真菌的跨境milRNAs可以准致病性真菌中的必需基因.
这项研究为开发用于预防和治疗真菌病的基于miRNA的新策略提供了基础.

关键词:

这是一种抗真菌药物.菌类寄生虫的真菌寄生主义跨境的千RNA是什么意思三皮虫 (Trichoderma) 是一种三皮虫.在sRNA测序过程中,

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