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循环miRNA-122与膝关节关节炎进展有关:雅库莫研究中的6年纵向队列研究

Hiroto Funahashi¹, Yasuhiko Takegami¹, Yusuke Osawa¹

¹Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan.

Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association

|November 9, 2023

概括

循环中的microRNAs (c-miRNAs) 可以预测膝关节骨关节炎 (KOA) 的进展. 具体而言,c-miRNA-122水平表明一般人群中KOA风险增加,有助于早期检测和管理.

科学领域:

生物化学生物化学
遗传学是一个遗传学.
整形外科整形外科整形外科

背景情况:

微RNAs (miRNAs) 参与了骨关节炎 (OA) 的发病过程.
预测膝关节骨关节炎 (KOA) 进展的miRNAs的纵向研究有限.

研究的目的:

确定与KOA进展相关的循环miRNAs (c-miRNAs).
评估c-miRNAs在一般人群中对KOA进展的预测效用.

主要方法:

从雅库莫研究 (2012-2018) 中对66名参与者 (128个膝盖) 的纵向分析.
在凯尔格伦-劳伦斯 (KL) 分类中以两级或更大的增长来定义的进展.
定量实时PCR选了21个c-miRNA;在渐进性和非渐进性OA组之间比较表达水平.

主要成果:

六种c-miRNAs (let7d,c-miRNA-122,150,199,21,320) 与KOA的进展有关.
c-miRNA-122独立地预测了KOA的进展 (OR:1.510,p=0.023).
ROC分析显示c-miRNA-122的AUC为0.702,用于预测OA进展.

结论:

c-miRNA-122表达水平与KOA进展风险有关.
c-miRNA-122显示出作为预测KOA进展的生物标志物的潜力.
这些发现与居住在社区的日本人群有关.

关键词:

循环中的微RNA.进行健康检查.膝关节骨关节炎是一种关节炎.纵向研究是一项纵向研究.风险因素风险因素

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...