在人类蛋白质组中,α-螺旋式阴离子两片段的发生和进化保护分析
Igor S D Costa1, Tiago Junot1, Fernanda L Silva1
1Laboratório de Síntese e Análise de Biomoléculas - LSAB, Instituto de Química, Universidade de Brasília, Brazil.
The FEBS journal
|November 9, 2023
概括
人类蛋白质含有具有抗微生物活性的加密碎片. 这些使用卡马尔软件识别的细分显示出作为抗微生物和抗癌剂的潜力,无论它们与细胞膜相关联.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 人类蛋白质内加密的片段表现出抗微生物活性.
- 最近的进展包括用于蛋白质组中这些细分的大规模识别的算法.
- 这些细分通常是阴性和两性,在合成时破坏细胞膜.
研究的目的:
- 通过使用Kamal软件,在人类参考蛋白质组内识别和表征假设的内基抗菌 (IAP).
- 评估这些片段的频率,父蛋白的功能类,结构相关性和进化保存.
- 为了评估选定的合成IAP序列的抗微生物和抗癌活性.
主要方法:
- 使用卡马尔软件对人类参考蛋白质组进行in silico分析,以识别cationic和amphiphilic部分.
- 细分特征的评估,包括频率,母蛋白功能,结构相关性和氨基酸残留的保存.
- 在体外评估六个合成的假定IAP序列的抗微生物和抗癌活性.
主要成果:
- 在2%的人类蛋白质中,主要在膜相关蛋白质中,发现了阴离子和两离子细分.
- 这些细分代表了表面暴露的基本斑块,其保存模式与其他表面残留物相似.
- 合成IAP序列显示出抗微生物和抗癌活性,独立于它们的原生膜协会.
结论:
- 人类蛋白质组含有加密的抗微生物 (IAP) 分段,特别是在膜蛋白内.
- 这些IAP具有固有的抗微生物和抗癌特性,表明潜在的治疗应用.
- 这些发现有助于理解这些细分的双重作用,即作为母蛋白的组成部分和独立的生物活性分子.
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