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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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广场内镜配件用于多重光成像.
Gaoming Li1, Miki Lee1, Tse-Shao Chang2
1Division of Gastroenterology, Department of Internal Medicine, University of Michigan, 109 Zina Pitcher Pl. BSRB 1522, Ann Arbor, MI, 48109-2200, USA.
Scientific reports
|November 9, 2023
概括
一种新的灵活的,纤维合的光内镜配件,可以同时检测多个分子标. 这项技术允许实时,体内成像,潜在地改善了空心器官疾病的检测.
科学领域:
- 医学内镜医学内镜
- 光学成像技术的成像
- 生物医学工程 生物医学工程
背景情况:
- 传统的白光内镜可以通过光成像来增强,同时检测多个分子标.
- 对于临床光成像,需要灵活的,与标准医疗内镜兼容的纤维合配件.
研究的目的:
- 为标准医疗内镜展示一种灵活的纤维合配件,能够收集光图像.
- 为了评估这种配件在体内多重光成像中的性能.
主要方法:
- 设计和制造了一个小型扫描镜 (1.30 × 0.45 mm2) 用于广角偏斜.
- 将扫描镜集成到直径为2.6毫米,长度为12毫米的远端,可实现实时,宽视野 (FOV) 图像生成.
- 在体内从小鼠采集多重光图像,使用3个以光标记的化物以每秒10的速度.
主要成果:
- 达到±27.4°和±22.8°的最大分歧角度,在10mm的工作距离下,FOV为10.4mm和8.4mm.
- 成功地将收集的光图像分成3个频道.
- 测量的目标与背景比为2.6,3.1和3.9.
结论:
- 开发的柔性纤维合光内镜配件适合临床使用.
- 这项技术显示了通过同时对多个分子点进行成像来检测空洞器官中的异质疾病的潜力.
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