与神经退行相关的蛋白质VAPB调节了脑髓母细胞瘤中的增殖
Amanda Faria Assoni1,2, Thiago Giove Mitsugi3, René Wardenaar4
1Human Genome and Stem Cell Research Center, Institute of Biosciences, University of São Paulo, 106, Rua do Matão, Cidade Universitária, São Paulo, 05508-090, Brazil. amandafassoni@gmail.com.
Scientific reports
|November 9, 2023
概括
在脑髓母细胞瘤中,高VAMP相关蛋白B和C (VAPB) 表达与生存率差相关. VAPB对于脑髓母细胞瘤细胞增殖至关重要,表明其作为治疗点的潜力.
科学领域:
- 神经瘤学神经瘤学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 囊泡相关膜蛋白B和C (VAPB) 与神经退行性疾病有关.
- 在瘤发生过程中,尤其是脑髓母细胞瘤中,VAPB的作用仍然在很大程度上未被探索.
- 脑髓母细胞瘤是一种儿科大脑瘤,起源于发育中的神经细胞.
研究的目的:
- 研究VAPB在脑髓母细胞瘤发育和进展中的作用.
- 确定VAPB表达与脑髓母细胞瘤患者存活率之间的相关性.
- 探索VAPB对脑髓母细胞瘤细胞增殖和细胞循环的影响.
主要方法:
- 在脑髓母细胞瘤患者样本中分析VAPB表达.
- 在体外和体内使用VAPB淘汰 (VAPBKO) 脑细胞母细胞瘤细胞的研究.
- 细胞周期进展分析.
- 在VAPBKO细胞中对WNT相关蛋白质的转录基因分析.
主要成果:
- 脑髓母细胞瘤组织中高VAPB表达与患者存活率降低有关.
- 在体外和体内,VAPB对于维持脑髓母细胞瘤细胞增殖率至关重要.
- VAPB淘汰导致细胞周期进展的延迟.
- VAPB淘汰导致WNT相关蛋白质的转录水平降低.
结论:
- 与VAMP相关的蛋白B和C (VAPB) 对于脑髓母细胞瘤细胞增殖至关重要.
- VAPB表达水平与脑髓母细胞瘤患者的预后相关.
- VAPB 代表了脑髓母细胞瘤治疗的潜在治疗标.
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