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鉴定circATG9A作为细胞癌的新生物标志物

Ye Shen¹, Ruipeng Wu^2,3, Zou Zhuo^2,3

¹Department of Urology, Northern Jiangsu People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, China. 18051063950@yzu.edu.cn.

Cancer gene therapy

|November 9, 2023

概括

循环RNAs (circRNAs) 越来越多地与癌症有关. 这项研究确定circRNA circATG9A是通过miR-497-5p/TRPM3途径促进细胞癌 (RCC) 进展的促进者,这表明它是治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

在全球范围内,细胞癌 (RCC) 的发病率和死亡率正在上升.
循环RNAs (circRNAs) 正在成为癌症进展的关键调节者.
了解RCC中的circRNA作用对于开发新疗法至关重要.

研究的目的:

在清细胞细胞癌 (ccRCC) 中识别差异表达的circRNAs.
研究一种新型circRNA,circATG9A在RCC进展中的作用和机制.
探索circATG9A作为RCC的潜在治疗点.

主要方法:

对ccRCC和正常组织的RNA测序.
通过RT-PCR,CCK-8,Transwell,伤口愈合和殖民地形成试验来研究circATG9A的功能.
为了阐明调节机制,进行了FISH,RNA pull-down, luciferase reporter和RIP测试.

主要成果:

确定了118种差异表达的circRNA,包括上调的circATG9A,它在体外和体内促进RCC.
确立了TRPM3作为circATG9A的标,由circATG9A海绵化miR-497-5p.p.介导.
已证实circATG9A通过Wnt/β-catenin通路促进表皮质-介质细胞过渡 (EMT).

结论:

circATG9A是RCC中的一种新型,上调的circRNA,可以驱动瘤的进展.
在RCC中,circATG9A/miR-497-5p/TRPM3轴调节EMT和Wnt/β-catenin信号传输.
circATG9A 是细胞癌的潜在诊断和治疗标.

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