诱导CtsR regulon可以改善Bacillus subtilis中西兰酶的产生
Biwen Wang1, Frans van der Kloet1, Leendert W Hamoen2
1Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, C3.108, 1098 XH, Amsterdam, The Netherlands.
Microbial cell factories
|November 10, 2023
概括
通过非激活CtsR抑制剂来打破Bacillus subtilis中Clp蛋白伴侣的负反调节,可显著增加25%的内分泌-1,4-β-xylanase XynA产量. 这一策略可能会提高其他生物体的酶产量.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 细菌细菌是因其蛋白质分泌能力而成为酶生产的关键工业宿主.
- 酶生产效率各不相同,优化仍然具有挑战性,通常依赖于试错.
- 对于B. subtilis.中的酶分泌存在有限的转录组研究.
研究的目的:
- 调查高水平内分泌-1,4-β-xylanase XynA表达对B. subtilis转录基因组的影响.
- 用全基因组转录组分析来识别分泌瓶.
- 探索改善酶生产产量的策略.
主要方法:
- 利用RNA测序 (RNA-seq) 进行全基因组转录组分析.
- 使用GINtool基因组分析工具来分析表达数据.
- 转基因B. subtilis通过非激活CtsR抑制剂来进行基因改造.
主要成果:
- 过度生产XynA导致CtsR regulon的活性降低,其中包括必不可少的蛋白质伴侣基因 (clpC,clpE,clpX).
- 确定了CtsR regulon的负反循环,涉及ClpC和ClpP调节的蛋白质分解.
- 不激活CtsR抑制器导致XynA产量增加了25%.
结论:
- 高水平的酶生产可以通过负反来耗尽B. subtilis中的Clp蛋白伴侣.
- 破坏这种反机制可以提高酶生产产量.
- 这些发现表明,由于保留的陪伴者系统,在其他微生物宿主中改善高产酶生产的潜在应用.
相关概念视频
01:25Inducible Operons: lac Operon
34
The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
34
01:17Gene Regulation During Sporulation
20
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
20
01:58Prokaryotic Transcriptional Activators and Repressors
21.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.1K