肠道微生物群组成,肠道感染和线性生长障碍之间的相关性:印度尼西亚阿切省Pidie的儿童发育迟缓的病例对照研究
Tristia Rinanda1,2, Catur Riani1, Anita Artarini1
1Department of Pharmaceutics, School of Pharmacy, Institut Teknologi Bandung, Ganesha 10, Bandung, 40132, West Java, Indonesia.
Gut pathogens
|November 10, 2023
概括
孩子 孩子 孩子 孩子
科学领域:
- 微生物学 微生物学
- 儿科 儿科 儿科
- 人类健康 人类健康 人类健康
背景情况:
- 儿童发育迟缓与环境肠道功能障碍 (EED) 和肠道失调有关.
- 印度尼西亚的肠道微生物组和肠道感染在衰老中的背景仍然未得到充分探索.
- 调查这些因素对于理解和解决儿童发展迟缓至关重要.
研究的目的:
- 为了确定肠道微生物群组成和儿童发育迟缓之间的相关性,在印度尼西亚阿切省Pidie.
- 评估肠道感染与生长生物标志物,特别是类似胰岛素的生长因子1 (IGF-1) 之间的关系.
- 为了确定与研究人口中衰老相关的特定微生物和遗传标记.
主要方法:
- 一项涉及42名24-59个月龄段儿童的病例对照研究 (21名发育迟缓,21名正常儿童).
- 使用ELISA量化IGF-1血清水平.
- 通过16S rDNA amplicon测序和使用qPCR的病毒性基因表达分析进行肠道微生物组概况.
主要成果:
- 与正常儿童相比,缩儿童的IGF-1水平明显较低 (p ≤0.001).
- 观察到明显的肠道微生物组特征,在缩儿童中,菌菌和特定属 (例如,Blautia,Collinsella) 的丰度更高.
- 从肠道病原体 (例如,大肠杆菌,沙门氏菌) 增加毒性基因的表达在缩儿童中被发现,与身高和IGF-1水平负相关.
结论:
- 阿切省Pidie的缩儿童表现出独特的肠道微生物组概况,其特点是失能,包括促炎和与饮食相关的种类.
- 在缩儿童中存在肠道病原体毒性基因,这表明它们在生长障碍中起着作用.
- 恢复肠道微生物群平衡对于支持线性生长和预防儿童发育迟缓至关重要.
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