在亚历山大病的老鼠模型中,定量扩散成像和基因型按性别相互作用
Nicholas A Stowe1, Ajay P Singh2, Brian R Barnett3
1Department of Radiology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA.
Magnetic resonance in medicine
|November 10, 2023
概括
神经元定向分散和密度成像 (NODDI) 揭示了亚历山大病 (AxD) 的大鼠模型中的微观结构性大脑变化. 这项研究确定了基因型特异性改变和神经病变发生的潜在基于性别的差异.
科学领域:
- 神经成像是一种神经成像.
- 神经科学是一个神经科学.
- 生物标志物发现发现
背景情况:
- 亚历山大病 (AxD) 的诊断和分类目前依赖于定性神经成像,由于临床症状重叠,难以区分亚型.
- 需要定量生物标志物来更好地了解AxD神经病变发生和识别不同的亚型.
研究的目的:
- 将神经元定向分散和密度成像 (NODDI) 应用于AxD的CRISPR-Cas9大鼠模型.
- 为了获得对 AxD 神经基质和大脑微观结构变化的定量见解.
- 为了确定 AxD. 的新的定量 NODDI 生物标志物.
主要方法:
- 多扩散加权成像 (DWI) 对年龄和性别匹配的AxD和野生型Sprague Dawley大鼠进行.
- 扩散张力成像 (DTI) 和NODDI测量为各种有趣的大脑区域计算.
- 一个3x2x2的ANOVA模型评估了基因型,生物学性别和横向性对DTI和NODDI措施的影响.
主要成果:
- 在杏仁体,海马体,新皮质和 thalamus 中观察到对 DTI 和 NODDI 测量的显著基因型影响.
- 在DTI和NODDI测量中,通过生物性相互作用发现了一个显著的基因型,该基因型位于体,海马体和新皮层内.
- 确定了白质道和皮层下灰色核的变化.
结论:
- 这项研究是第一个在亚历山大病的遗传大鼠模型中利用NODDI的研究.
- 量化DTI和NODDI测量显示了 AxD.中的显著微观结构性大脑变化.
- 通过性别相互作用确定的基因型表明生物性别在AxD神经病变发生过程中的潜在作用.
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