相关实验视频

Updated: Jul 11, 2025

Isolation and Enrichment of Rat Mesenchymal Stem Cells MSCs and Separation of Single-colony Derived MSCs

Isolation and Enrichment of Rat Mesenchymal Stem Cells MSCs and Separation of Single-colony Derived MSCs

Published on: March 22, 2010

评估从介质干细胞制成的条件介质对大鼠精子干细胞分化的影响

Hoda Fazaeli¹, Mohsen Sheykhhasan¹, Naser Kalhor¹

¹Department of Mesenchymal Stem Cells, Academic Center for Education, Culture, and Research (ACECR), Qom Branch, Qom, Iran.

Anatomy & cell biology

|November 10, 2023

概括

脂肪组织衍生中干细胞条件介质促进精子干细胞 (SSC) 分化,为化疗诱导的不孕症提供潜在的治疗方法. 这项研究表明,来自AD-MSCs的条件介质可以在体外诱导SSC分化.

科学领域:

生殖生物学生殖生物学
干细胞生物学干细胞生物学
瘤科支持性护理

背景情况:

化疗和放射治疗可以通过破坏精子生成和影响精子干细胞 (SSCs) 来引起不孕症.
干细胞的体外分化,特别是介质干细胞 (MSCs),成生殖细胞是治疗不孕症的一个有希望的策略.
脂肪组织衍生MSCs (AD-MSCs) 为生成条件介质 (CM) 提供了一个易于获取的来源.

研究的目的:

评估SSCs的增殖和分化,当与AD-MSCs的Sertoli细胞和条件介质 (CM) 共同培养时.
调查AD-MSC衍生的CM作为诱导SSC分化的生物材料的潜力.
评估AD-MSCCM治疗后关键精子生成标记物的表达.

主要方法:

从新生Wistar老鼠中分离和共同培养SSC和塞尔托利细胞.
对共培养的细胞进行12天的AD-MSCsCM治疗.
使用RT-qPCR和免疫细胞化学测量与差异化相关的基因表达 (Scp3,Dazl,Prm1,Stra8),以及对DAZL和DDX4蛋白水平的西方涂抹.

主要成果:

与对照组相比,在AD-MSCCM治疗后,Scp3,Dazl和Prm1基因表达显著增加.
在实验组中,对DAZL和DDX4的免疫细胞化学染色结果呈阳性.
西部涂抹在治疗组中显示了DAZL和DDX4的更高表达,尽管在统计学上并不显著.

结论:

来自AD-MSCs的条件介质可以在体外诱导SSCs的分化.
AD-MSC CM显示出作为不孕症治疗策略的生物材料的潜力.
需要进一步的研究来优化条件,并确认AD-MSCCM在促进精子生成方面的有效性.

关键词:

有条件的介质.不同化的差异化介质细胞干细胞介质细胞干细胞精子干细胞 (Spermatogonial stem cells) 是一种精子干细胞.

更多相关视频

Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines

Serial Enrichment of Spermatogonial Stem and Progenitor Cells SSCs in Culture for Derivation of Long-term Adult Mouse SSC Lines

Published on: February 25, 2013

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...