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微纳米通过激活与衰老相关的分泌表现型来加速介质干细胞的衰老

Xuan Li¹, Xinxin Luo¹, Ye He²

¹Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University Chongqing 400044, P. R. China.

|November 10, 2023

概括

由于干细胞衰老,微纳米 (MNT) 植入物不能改善老老鼠的骨质整合. 在老年患者中,MNT加剧衰老,耗尽干细胞并阻碍骨愈合.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
老年学是一门学科.

背景情况:

干细胞衰老是生物体衰老的关键因素,可能会损害骨科治疗.
干细胞的衰老可能会损害植入物的成功,导致潜在的失败.
了解植入物表面上的干细胞行为对于改善老年人群的骨科结果至关重要.

研究的目的:

研究干细胞衰老对微纳米 (MNT) 植入物的骨质整合的影响.
在体内和体外探索MNT影响骨髓衰老的骨髓介质干细胞 (BMSCs) 的机制.
为设计为老年患者量身定制的骨科植入物提供见解.

主要方法:

微纳米 (MNT) 的制造和表征.
在MNT上使用老化和正常骨髓介质干细胞 (BMSCs) 的体外和体内研究.
在老老鼠中分析干细胞粘附,骨质分化,衰老标记 (p21),NF-κB通路和SASP.

主要成果:

与正常大鼠相比,MNT在老老鼠中没有显著增强骨质整合.
通过p38 MAPK激活,MNT促进了NF-κB在衰老的BMSC中的核定位,诱导SASP并耗尽干细胞池.
虽然MNT激活了p38 MAPK,但它仍然在非衰老的BMSC中促进了骨质分化,这表明了矛盾的效应.

结论:

干细胞衰老显著损害了MNT植入物在老年人中的骨质整合.
MNT可以加剧干细胞的衰老,导致植入物接口的干细胞池减少.
这些发现突出了MNT表面的骨质生成和衰老之间的复杂相互作用,这对于开发用于老年患者的植入物至关重要.

关键词:

介质细胞干细胞介质细胞干细胞微纳米结构微纳米结构骨质整合骨质整合衰老是一种老化.的植入物是可以植入的.

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