在固态发酵下使用Syzigium cumini叶片净化和表征来自Aspergillus fumigatusMA的细胞外酶
Krishan Kumar Selwal1, Manjit K Selwal1
1Department of Biotechnology, DCR University of Science and Technology, Sonepat, Haryana, India.
Preparative biochemistry & biotechnology
|November 10, 2023
概括
这项研究使用固态发酵和染色学净化了来自Aspergillus fumigatus的酶. 该酶表现出最佳的活性和稳定性,表明其在食品,料和制药应用中的潜力.
科学领域:
- 生物化学 生物化学
- 酶技术 酶技术是一种
- 工业微生物学 工业微生物学
背景情况:
- 酶酶对于化素至关重要.
- 制厂废水中的真菌分离物为酶生产提供了可持续的来源.
- 固态发酵 (SSF) 是微生物酶生产的有效方法.
研究的目的:
- 为了净化来自Aspergillus fumigatusMA的酶.
- 为了描述纯化的酶酶.
- 评估化剂在各种工业中的潜在应用.
主要方法:
- 固体发酵 (SSF) 用于化酶的生产.
- 硫酸沉和DEAE-纤维素离子交换色谱用于净化.
- 用于分子质量测定的SDS-PAGE.
- 在不同的pH值和温度下进行酶活性和稳定性测试.
主要成果:
- 坦纳经过23倍丰富和27.6%回收的净化.
- 纯化的酶的分子质量为66.2kDa.
- 在pH值5.0-6.0和30°C时观察到最佳活性.
- 该酶在广泛的pH范围 (4.0-7.0) 和温度范围 (30-50°C) 中表现出稳定性.
- 确定了运动参数 (Km和Vmax).
结论:
- 阿斯珀吉勒斯烟虫MA是一种可行的酸酶生产来源.
- 纯化酸具有有利的生化特性,可用于工业用途.
- 该酶的特性表明在食品,料和制药行业具有重大潜力.
相关概念视频
Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...


