相关实验视频
Updated: Jul 11, 2025

10:40
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
2.4K
ceRNA网络分析揭示了COVID-19相关慢性阻塞性肺病中的潜在关键miRNA和向基因
Lihai Zhang1, Xiaodong Jia2, Zhipeng Zhang3
1Department of Respiratory and Critical Care, The First Hospital of Hebei Medical University, No.89, Donggang Road, Shijiazhuang City, 050000, Hebei Province, China.
Applied biochemistry and biotechnology
|November 10, 2023
概括
这项研究揭示了关键的基因和microRNA调节器,涉及SARS-CoV-2感染和慢性阻塞性肺病 (COPD) 之间的相互作用. 这些发现为患病患者的早期干预提供了潜在的预后生物标志物.
科学领域:
- 基因组学和生物信息学
- 呼吸系统医学 呼吸系统医学
- 传染性疾病 传染性疾病
背景情况:
- 全球SARS-CoV-2的传播给公共卫生带来了重大挑战,尤其是慢性阻塞性肺病 (COPD) 患者.
- 确切的SARS-CoV-2影响宿主基因表达,COPD进展和患者预后的机制尚不清楚.
研究的目的:
- 通过整合来自COPD患者的差异性基因表达数据,构建一个COPD特定的竞争性内源性RNA (ceRNA) 网络.
- 分析与SARS-CoV-2相关的差异表达基因 (DEG),微RNA (miRNA) 和它们的标之间的相互作用.
- 确定关键的监管关系和网络模块,以了解COPD中SARS-CoV-2感染的功能机制.
主要方法:
- 来自COPD患者和SARS-CoV-2感染个体的综合差异基因表达数据.
- 计算了mRNA-miRNA相关性,以建立一个COPD特定的ceRNA网络.
- 从9个SARS-CoV-2序列中分析了DEG,miRNA和目标;探索了网络模块.
主要成果:
- 较高比例的DEG在同一miRNA中竞争,这表明COPD ceRNA网络内的基因表达增加.
- Hsa-miR-21-3p是一个高度连接的节点,但其对基因上调调节的调节影响很低,这表明高表达是COPD的不良预后因素.
- Hsa-miR-15a-5p是最重要的miRNA,与ANXA2P3.3相互作用. 涉及hsa-miR-1184和hsa-miR-99的模块,与相互作用的基因ZDHHC18,PCGF3和KIAA0319L,是显著的,并与不良的COPD预后有关.
结论:
- 包括miR-21,miR-15a,ANXA2P3,ZDHHC18,PCGF3和KIAA0319L在内的关键调节剂被确定为潜在的预后生物标志物.
- 这些生物标志物可以为与SARS-CoV-2同时感染的COPD患者提供早期干预策略.
相关概念视频
Chronic Obstructive Pulmonary Disease-II: Pathophysiology
2.8K
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Chronic Inflammation
2.8K
Chronic Obstructive Pulmonary Disease-I: Introduction
2.8K
Chronic Obstructive Pulmonary Disease (COPD) is a long-lasting respiratory condition requiring continuous attention and care. It is a progressive lung disease that leads to breathing challenges due to airflow obstruction. It manifests as persistent respiratory symptoms and restricted airflow resulting from abnormalities in the airways and alveoli, usually due to long-term exposure to harmful particles or gases. COPD mainly consists of two primary conditions: emphysema and chronic bronchitis.
2.8K
Single Nucleotide Polymorphisms-SNPs
15.2K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.2K
Chronic Obstructive Pulmonary Disease
1.3K
COPD is defined as a heterogeneous lung condition marked by persistent respiratory symptoms such as dyspnea, cough, and sputum production, caused by abnormalities in the airways that cause airflow obstruction.
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...
1.3K
Chronic Obstructive Pulmonary Disease-III: Symptoms and Complications.
2.8K
Understanding the variety of primary symptoms and systemic complications that characterize chronic obstructive pulmonary disease (COPD) is crucial for healthcare professionals.
Symptoms of COPD can be classified as primary or systemic. Primary symptoms relate to reduced airflow, while systemic or extrapulmonary symptoms relate to COPD's broader impact on the body.
Primary Symptoms of COPD:
Symptoms of COPD can be classified as primary or systemic. Primary symptoms relate to reduced airflow, while systemic or extrapulmonary symptoms relate to COPD's broader impact on the body.
Primary Symptoms of COPD:
2.8K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K

