PET/NIR光双模成像用于向瘤检测
Xin Xue1, Qiyi Li2, Pengjun Zhang3
1Department of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, Jiangsu China.
Molecular pharmaceutics
|November 10, 2023
概括
一个新的双模探头,[68Ga]Ga-DOTA-NPC,增强了结肠癌的检测和手术指导. 这种成像剂结合了正子发射断层扫描 (PET) 和近红外光 (NIRF) 以改善瘤可视化和切除.
科学领域:
- 在瘤学瘤学.
- 医疗成像医学成像
- 放射化学 放射化学是指辐射化学.
背景情况:
- 癌症诊断和治疗受到晚期检测和瘤不完全切除的挑战.
- 整合正子发射断层扫描 (PET) 和近红外光 (NIRF) 的双方式探针为全面的癌症管理提供了潜力.
- 针对特定的癌细胞机制,如有机阴离子载体聚1B3 (OATP1B3),可以提高成像剂的特异性.
研究的目的:
- 开发和评估一种新的PET/NIRF双模成像剂[68Ga]Ga-DOTA-NPC,用于结肠癌检测和外科指导.
- 评估探针对表达OATP1B3.3.的结肠癌细胞的特异性.
- 调查[68Ga]Ga-DOTA-NPC在瘤成像和切除方面的体外和体内性能.
主要方法:
- 合成PET/NIRF双模探头[68Ga]Ga-DOTA-NPC,通过通过乙二和放射性标记用-68.8将DCDSTCY和DOTA合起来.
- 在体外对结肠癌细胞中探针吸收的评估.
- 在实体研究中,使用动物的 ортотоп和皮下结肠瘤模型进行PET/NIRF成像和手术指导.
主要成果:
- 探测器[68Ga]Ga-DOTA-NPC证明了结肠癌细胞的优先吸收,这归因于OATP1B3的表达.
- PET/NIRF成像证实了高对比度瘤积累和探针的良好生物安全性.
- 在实时NIRF指导下,成功切除了正位体和皮下结肠瘤.
结论:
- [68Ga]Ga-DOTA-NPC是一种有前途的双模成像剂,用于敏感,高对比度检测结肠癌.
- 该探针可提供实时的手术内外科手术指导,以完成瘤切除.
- [68Ga]Ga-DOTA-NPC提供先进的PET/NIRF成像功能,用于区分瘤与周围组织.
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