相关实验视频

Updated: Jul 11, 2025

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

IL-27通过增强葡萄糖吸收和脂肪酸化来增加白色脂肪细胞中的能量储存

Chiara Scaffidi^1,2, Annie Srdic¹, Daniel Konrad^1,2,3

¹Division of Pediatric Endocrinology and Diabetology, University Children's Hospital, University of Zurich, Zurich, Switzerland.

|November 10, 2023

概括

介素-27 (IL-27) 令人惊的是减少了自由脂肪酸,但增加了葡萄糖的吸收和三糖沉积在白色脂肪细胞. 这表明IL-27在这些细胞中促进能量储存而不是消散.

科学领域:

细胞生物学细胞生物学
代谢过程中的代谢.
内分泌学在内分泌学.

背景情况:

众所周知,互乐金-27 (IL-27) 在白色脂肪细胞中诱导热生成.
IL-27对这种热生成的能量供应基质 (脂质和碳水化合物) 的影响尚不清楚.
以前的假设表明IL-27可能会增加脂解和葡萄糖的吸收,以燃料能量消耗.

研究的目的:

为了调查IL-27诱导的白脂肪细胞热生成是否伴随着脂解和葡萄糖吸收的增加.
阐明IL-27影响脂肪细胞中的脂质和碳水化合物代谢的机制.

主要方法:

用IL-27.7治疗3T3-L1脂肪细胞和初级皮下白脂肪细胞.
测量细胞内和细胞外自由脂肪酸 (FFA) 度.
对激素敏感脂酶 (HSL) 酸化的评估.
细胞内甘油三酸 (TG) 含量的量化.
分析线粒体ATP的产生和β-氧化酶的表达.
评估GLUT1蛋白水平和基底葡萄糖摄取量.
测量糖溶性ATP的产生.

主要成果:

治疗IL-27可以降低脂肪细胞中的细胞内和细胞外FFA度.
HSL酸化没有受到IL-27的改变,细胞内TG含量增加.
IL-27提高了GLUT1蛋白水平,基底葡萄糖吸收和糖分 ATP 生产,但不是线粒体 ATP 或β氧化.
研究结果表明,脂肪酸的化增加和增强的糖解有助于观察到的代谢变化.

结论:

与最初的假设相反,IL-27不会增加脂解,而是促进白色脂肪细胞中的甘油三合成.
IL-27通过糖解来增强葡萄糖的吸收和利用,为甘油三合成提供基质.
这些发现表明,IL-27通过葡萄糖吸收和白色脂肪细胞中的甘油三沉积促进能量储存.

关键词:

自由脂肪酸是一种自由的脂肪酸.脂肪溶解是指脂质分解的过程.肥胖肥胖肥胖肥胖肥胖肥胖肥胖肥胖甘油三胺的使用方法这是白色脂肪组织.

更多相关视频

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

Isolation of Adipose Tissue Immune Cells

Isolation of Adipose Tissue Immune Cells

Published on: May 22, 2013

相关概念视频

Hormones Regulating Blood Glucose

Hormones Regulating Blood Glucose

Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...

Insulin Secretory Vesicles

Insulin Secretory Vesicles

Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...

Insulin: The Receptor and Signaling Pathways

Insulin: The Receptor and Signaling Pathways

Insulin action is mediated through a receptor tyrosine kinase, akin to the IGF-1 receptor. The number of receptors per cell varies significantly, from 40 on erythrocytes to 300,000 on adipocytes and hepatocytes. The insulin receptor consists of linked α/β subunit dimers, forming a heterotetramer glycoprotein with two extracellular α subunits and two β subunits spanning the membrane. The α subunits inhibit the inherent tyrosine kinase activity of the β subunits, but...