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仙人掌 (Anthriscus sylvestris) 脱氧叶毒素合成酶参与叶毒素生物合成

Keisuke Kobayashi¹, Masaomi Yamamura^1,2, Bunzo Mikami¹

¹Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto, 611-0011 Japan.

Plant & cell physiology

|November 10, 2023

概括

研究人员发现了一种新的酶,Anthriscus sylvestris脱氧基毒素合成酶 (AsDPS),来自牛. 这种Fe (II) -和2-氧格酸盐依赖的二氧化酶 (2-ODD) 酶对于合成抗癌干素 (如 podophyllotoxin) 至关重要.

科学领域:

生物化学生化学
植物科学植物科学
酶学是一种酶学.

背景情况:

甲基酸环的形成是阿里甲林生物合成的关键,包括脱氧基毒素和基毒素.
来自Podophyllum hexandrum的脱氧胺合成酶 (DPS) 催化了 yatein 循环,但DPS 进化是跨无关植物物种的谱系特异性的.

研究的目的:

为了识别和表征来自Anthriscus sylvestris (AsDPS) 的脱氧叶毒素合成酶 (DPS).
了解各种植物物种中DPS的酶特性和进化见解.

主要方法:

在2-ODD超级家族中对AsDPS的家族遗传分析.
复合蛋白表达和酶分析以确定基质选择性.
蛋白质建模 (AlphaFold2) 和位点定向突变发生,以确定关键残留物.

主要成果:

AsDPS,一个2-ODD酶,从A. sylvestris.中被识别和表征.
AsDPS与P. hexandrum DPS (PhDPS) 具有中度的序列相同性,但表现出不同的基质反体选择性.
在AsDPS中的Tyr305残留物被确定为可能对基质识别至关重要.

结论:

这项研究阐明了在A. sylvestris.中波多菲洛毒素生物合成途径.
它为植物二次代谢中的2-ODD酶的功能多样性提供了新的见解.
这些发现强调了研究不同植物系的DPS的重要性,以全面了解干生物合成.

关键词:

这种植物是Anthriscus sylvestris.这是一种依赖于二氧化酸酸盐的二氧化酶.脱氧波多菲洛毒素合成酶是什么?在Lignan和Lignan之间.波多菲洛托克辛 (podophyllotoxin) 是一种可怕的毒素.在Yatein中,我们可以使用Yatein.

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