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相关概念视频

Overview of Exosomes01:36

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Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
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Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
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对外体捕获和分析外体微RNA的功能表面.

Cristina Potrich1, Anna Pedrotti2, Cecilia Pederzolli2

  • 1FBK-Fondazione Bruno Kessler, Center for Sensors and Devices, via Sommarive, 18, I-38123, Trento, Italy; Consiglio Nazionale delle Ricerche, Istituto di Biofisica, via alla Cascata 56/C, I-38123, Trento, Italy.

Colloids and surfaces. B, Biointerfaces
|November 10, 2023
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概括

研究人员开发了新的功能化表面,以有效地隔离外体. 在4x10^8外体/cm2上捕获的负电荷表面,可用于生物传感器的微RNA生物标志物分析.

关键词:
其他 - - miR-21外体体捕获外体的捕获这是一个微型RNA.实时PCR可以实现.表面功能化的功能化.

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科学领域:

  • 生物技术是生物技术.
  • 纳米技术 纳米技术
  • 分子生物学分子生物学

背景情况:

  • 外体对细胞信号传递至关重要,并含有诸如microRNA等有价值的生物标志物.
  • 目前的外体隔离方法的效率和纯度都很低.
  • 微流体和功能化表面为改善隔离提供了潜在的解决方案.

研究的目的:

  • 开发和表征功能化的表面,以有效地捕获外体.
  • 评估不同表面化学物质对外体隔离的性能.
  • 为了实现芯片上的外体隔离,用于生物标志物分析.

主要方法:

  • 氧化基板具有充电 (正,负,中性) 和免疫 afinity 表面的功能化.
  • 测试表面具有外体体模仿囊泡和纯化的外体体.
  • 使用RT-PCR对被捕获的外体细胞进行量化和对外体微RNA的分析.

主要成果:

  • 负电荷的表面显示出高的外体细胞捕获能力 (>4 × 10^8外体细胞/cm2).
  • 捕获的外体细胞保留了它们的完整性,用于生物标志物分析.
  • 从捕获的外体细胞中成功对外体微RNA进行RT-PCR分析.

结论:

  • 功能化表面,特别是负电荷的表面,显著提高了外体隔离效率和纯度.
  • 开发的表面适合集成到微流体设备中,用于芯片上的外体隔离.
  • 这种方法有助于开发用于临床和研究生物标志物分析的快速生物传感器.