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Updated: Jul 11, 2025

Identification of protein complexes with quantitative proteomics in S. cerevisiae
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利用内源性酵母核蛋白质来识别短线性动机 in vivo

Tanner M Tessier1, Cason R King1, Joe S Mymryk2

  • 1Department of Microbiology and Immunology, Western University, London, ON, Canada.

Cell reports methods
|November 10, 2023
PubMed
概括

我们开发了一种基于酵母的功能选择,以发现与核蛋白质组相互作用的. 这些已识别的短线性动图 (SLiM) 也介导了人类细胞中的核进口,揭示了新的进口途径.

关键词:
CP: 细胞生物学 细胞生物学科普:分子生物学 分子生物学遗传选择 遗传选择模型生物模型生物.非传统核进口非传统核进口背着一个小猪背着一个小猪背.蛋白质与蛋白质的相互作用一个简短的线性图案.合成生物学 合成生物学

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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