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在体外器官培养的胎儿丸中,uron诱导的胎儿莱迪格细胞功能障碍

Ran Lee¹, Won-Young Lee², Hyun-Jung Park³

¹Department of Livestock, Korea National College of Agriculture and Fisheries, Jeonbuk 54874, Republic of Korea; Department of Animal Biotechnology, Sangji University, Wonju-si, Gangwon-do 26339, Republic of Korea.

Reproductive toxicology (Elmsford, N.Y.)

|November 10, 2023

概括

迪乌龙暴露对小鼠胎儿莱迪格细胞发育产生负面影响. 然而,这种常见的除草剂不会影响胎儿胚胎或塞尔托利细胞,这表明特定的毒性途径.

科学领域:

生殖毒理学生殖毒理学
内分泌学在内分泌学.
发展生物学发展生物学

背景情况:

环境污染物,包括uron等除草剂,对生殖健康构成风险,并可能导致发育缺陷.
类除草剂在农业中广泛使用,增加了人类和环境的潜在暴露.

研究的目的:

为了研究uron对胎儿小鼠丸发育的特定毒性作用.
在体外模型中评估迪乌伦对生殖细胞,塞尔托利细胞和莱迪格细胞分化的影响.

主要方法:

利用胎儿小鼠丸的三维器官培养 (胚胎日14.5).
培养丸暴露在200μM的迪乌龙中5天.
分析了生殖细胞增殖和生殖细胞/塞尔托利细胞标记物的表达 (例如,Ddx4,Sox9);评估了胎儿莱迪格细胞 (FLC) 和成人莱迪格细胞 (ALC) 标记物.

主要成果:

迪乌龙没有影响胎儿生殖细胞的增殖或关键生殖细胞和塞尔托利细胞标记物的表达.
迪乌龙暴露增加了胎儿莱迪格细胞 (FLC) 标记物 (Cyp11a1,Cyp17a1) 和减少了成人莱迪格细胞 (ALC) 标记物 (Sult1e1,Hsd173).
在FLC和ALC中共同的标志物3-βHSD保持稳定,表明莱迪格细胞成熟的特定干扰.

结论:

迪乌龙暴露特别扰乱了小鼠丸中胎儿莱迪格细胞的正常发育.
在测试条件下,生殖细胞和塞尔托利细胞似乎对二氨酸毒性有弹性.
这些发现凸显了迪乌龙在发育过程中干扰男性生殖内分泌功能的潜力.

关键词:

发展发展发展发展发展杀草剂是一种除草剂.莱迪格细胞的发育. 生殖性毒性生殖性毒性器官培养器官培养器官培养器官

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