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Updated: Aug 16, 2026

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
14:58

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Published on: November 12, 2012

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20kDa的辅助蛋白质 (P20) 来自Bacillus thuringiensis亚种中的. 以色列 ISPC-12:净化,表征,溶液散射和结构分析

Omkar U Kinkar1, Rahul Singh1, Arpit Prashar2

  • 1Homi Bhabha National Institute, Anushaktinagar, Mumbai 400094, Maharashtra, India; Beamline Development and Application Section, Bhabha Atomic Research Centre, Mumbai 400085, Maharashtra, India.

International journal of biological macromolecules
|November 10, 2023
PubMed
概括

甲杆菌的辅助蛋白P20充当分子伴侣,增强毒素的产生和杀虫活性. 这项研究描述了P20,揭示了它在细胞分解毒素家族中的二维结构和作用.

关键词:
细菌 thuringiensis 亚种. 这种类型的. 以色列人的以色列ensis.标志性描述 标志性描述细胞分解性 (Cyt) 毒素P20 P20 是一个很好的方法.结构建模的结构建模.在X射线中,散射是X射线的散射.

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Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis09:26

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相关概念视频

SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...